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脂肪酶催化合成异维生素C棕榈酸酯及其动力学
引用本文:徐凤杰,谭天伟.脂肪酶催化合成异维生素C棕榈酸酯及其动力学[J].北京化工大学学报(自然科学版),2007,34(Z2):95-98.
作者姓名:徐凤杰  谭天伟
作者单位:北京化工大学:1生命科学与技术学院;2北京市生物加工过程重点实验室,北京 100029
基金项目:国家重点基础研究发展计划(973计划) , 国家自然科学基金
摘    要:对来自于Candida sp.99-125的脂肪酶在有机溶剂中催化合成异维生素C棕榈酸酯反应进行了研究。结果表明,初始反应速率v0与棕榈酸和异维生素C摩尔浓度的比值有关。v0随着cpal/ciso的增大先是增大,至cpal/ciso值接近15时v0基本不变。另外,考察了溶剂、温度、转速、酶量、初始水含量对酶促反应的影响。研究表明该反应的反应机理很好的符合乒乓反应模型,确定了最反应速率vm和米氏常数k′m,由双倒数曲线可以得到,vm=5.86μmol/(min·g),k′m=0.092mmol/L。

关 键 词:异维生素C棕榈酸酯  固定化脂肪酶  丙酮  脂肪酶催化合成  异维生素  棕榈酸酯  动力学  esterification  palmitate  formation  曲线  双倒  米氏常数  初始反应速率  反应模型  乒乓  反应机理  影响  酶促反应  水含量  转速  温度  溶剂
收稿时间:2007-01-04
修稿时间:2007年1月4日

Kinetics of formation of isoascorbyl palmitate by lipase-catalyzed esterification
XU FengJie,TAN TianWei.Kinetics of formation of isoascorbyl palmitate by lipase-catalyzed esterification[J].Journal of Beijing University of Chemical Technology,2007,34(Z2):95-98.
Authors:XU FengJie  TAN TianWei
Institution:1College of Life Science and Technology; 2Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China
Abstract:The kinetics of the esterification reaction of isoascorbic acid with palmitic acid catalyzed by lipase from Candida sp. 99-125 have been studied in different organic solvents. The results showed that the initial rate v0 is related to the ratio of molar concentrations, cpal/ciso. The value of v0 initially increases with cpal/ciso and then reaches a maximum and remains constant at that value as cpal/ciso is increased further. The highest initial rate was obtained in acetone as solvent. The effects of varying temperature, shaking speed, initial water content and lipase concentration on the initial rate were investigated. On the basis of the kinetic model obtained, the reaction mechanism can be described by a Ping Pong model with vm=5.686μmol/(min·g), and k'm=0.092mmol/L. 
Keywords:isoascorbyl palmitate  immobilized lipase  acetone
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