| 胞壁结合型生物破乳菌Alcaligenes sp. S-XJ-1氮源利用特性 |
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| 引用本文： | 黄翔峰,倪晓静,彭开铭,刘佳,陆丽君.胞壁结合型生物破乳菌Alcaligenes sp. S-XJ-1氮源利用特性[J].同济大学学报(自然科学版),2013,41(6):856-861. |
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| 作者姓名： | 黄翔峰 倪晓静 彭开铭 刘佳 陆丽君 |
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| 作者单位： | 同济大学环境科学与工程学院,上海200092;同济大学污染控制与资源化研究国家重点实验室,上海200092 |
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| 基金项目： | 国家自然科学基金，教育部新世纪优秀人才支持计划，中央高校基本科研业务费专项资金 |
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| 摘 要： | 以六种氨基酸及硝酸铵为氮源培养胞壁结合型生物破乳剂产生菌Alcaligenes sp.S-XJ-1,进而分析菌体对谷氨酸和硝酸铵的利用过程,并建立菌体的生长及氮源利用模型.结果表明:氮源种类和投量是影响菌体产量和性能的重要因素;菌体对氮源的利用顺序依次为有机氮、氨态氮、硝态氮;谷氨酸为氮源时对生物破乳菌产量的促进效果最佳,相比于硝酸铵为氮源时,菌体最大比增长速率和产率系数更高,用于菌体代谢供能的氮源消耗量更低.
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| 关 键 词： | Alcaligenes sp. 氨基酸 氮源 生物破乳菌 |
| 收稿时间： | 8/2/2012 12:00:00 AM |
| 修稿时间： | 2013/3/18 0:00:00 |
Characteristics of Nitrogen Sourceutilization for Cell-bound Biodemulsifying Strain of Alcaligenes sp. S-XJ-1 |
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| Huang Xiangfeng,Ni Xiaojing,Peng Kaiming,Liu Jia and Lu Lijun.Characteristics of Nitrogen Sourceutilization for Cell-bound Biodemulsifying Strain of Alcaligenes sp. S-XJ-1[J].Journal of Tongji University(Natural Science),2013,41(6):856-861. |
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| Authors: | Huang Xiangfeng Ni Xiaojing Peng Kaiming Liu Jia and Lu Lijun |
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| Institution: | College of Environmental Science & Engineering, Tongji University, Shanghai 200092, China; State Key Laboratory of Pollution Control and Resource Reuse,Tongji University, Shanghai 200092, China;College of Environmental Science & Engineering, Tongji University, Shanghai 200092, China; State Key Laboratory of Pollution Control and Resource Reuse,Tongji University, Shanghai 200092, China;College of Environmental Science & Engineering, Tongji University, Shanghai 200092, China; State Key Laboratory of Pollution Control and Resource Reuse,Tongji University, Shanghai 200092, China;College of Environmental Science & Engineering, Tongji University, Shanghai 200092, China; State Key Laboratory of Pollution Control and Resource Reuse,Tongji University, Shanghai 200092, China |
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| Abstract: | Six animo acids and ammonium nitrate were tested to study the effects of nitrogen source on the growth of cell bound biodemulsifying strain of Alcaligenes sp. SXJ1. And then the utilization process of nitrogen source was studied when glutamic and ammonium nitrate was separately used as nitrogen source. The growth model and nitrogen utilization model of SXJ1 were built up as well. The results show that SXJ1 prefers to use organic nitrogen when organic nitrogen and ammonium nitrogen coexist in the substrate, and ammonium nitrogen is preferred rather than nitrate nitrogen. Also, the yield and property of biodemulsifying bacteria were significantly affected by the variety of nitrogen source,and glutamic is testified to be the best nitrogen source for SXJ1 among the six amino acids. When glutamic is used as nitrogen source, SXJ1 exhibits a higher maximum specific microbial incremental rate and utilization ratio of nitrogen source, but a lower energy cost. |
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| Keywords: | Alcaligenes sp amino acid nitrogen source demulsifying strain |
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