| 地衣芽孢杆菌1.934培养条件的优化 |
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| 引用本文： | 于淑玉,张光明,万甜.地衣芽孢杆菌1.934培养条件的优化[J].内蒙古大学学报(自然科学版),2012,43(3):278-282. |
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| 作者姓名： | 于淑玉 张光明 万甜 |
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| 作者单位： | 哈尔滨工业大学市政环境与工程学院,哈尔滨,150090 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 研究了生物肥功能菌——地衣芽胞杆菌1.934 (Bacillus licheniformis)培养条件对菌体生长量的影响.采用了单因素实验和响应曲面法(RSM)设计实验和分析数据.获得了菌体摇瓶培养的最适条件:培养温度35℃,转速为150r/min,接种量为3.6％,pH为7.5.地衣芽孢杆菌在最适条件下培养约20h,淀粉酶的酶活最高,活力可达12.7U/mL培养液.培养约27h得到最大的菌体收益.
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| 关 键 词： | 也衣芽孢杆菌 复合中心实验设计 淀粉酶 |
Optimization of Culture Condition for Bacillus licheniformis 1.934 Production |
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| YU Shu-yu
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ZHANG Guang-ming
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WAN Tian.Optimization of Culture Condition for Bacillus licheniformis 1.934 Production[J].Acta Scientiarum Naturalium Universitatis Neimongol,2012,43(3):278-282. |
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| Authors: | YU Shu-yu ZHANG Guang-ming WAN Tian |
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| Institution: | (School of Municipal and Environmental Engineering, Harbin Institute of Technology,Harbin 150090,China) |
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| Abstract: | Bacillus licheniformis is very important as a soil microorganism to increase the availability and uptake of mineral nutrients for plants.In this study,the effect of culture condition on Bacillus licheniformis 1.934 production was investigated.On the basis of single factor experiments,a central composite design was used to optimize the prime factors.The results were analyzed by response surface.Analysis results show that the optimal culture condition is as follows:temperature 35℃,flask shaking speed 150r/min,amount of inoculation 3.6%,pH 7.5.Bacillus licheniformis 1.934 grew well under this condition,incubated for 20h,the highest amylase activity(12.7U /mL) can be obtained;incubated for 27h,the highest cell number can be gotten. |
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| Keywords: | Bacillus licheniformis central composite design amylase |
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