| 胶束增敏荧光光谱法测定齐墩果酸的含量 |
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| 引用本文： | 闫丽丽,毕淑云,庞博,王羽.胶束增敏荧光光谱法测定齐墩果酸的含量[J].内蒙古大学学报(自然科学版),2012,43(2):137-140. |
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| 作者姓名： | 闫丽丽 毕淑云 庞博 王羽 |
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| 作者单位： | 长春师范学院化学学院,长春,130032 |
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| 基金项目： | 吉林省教育厅“十二五”科研规划项目(批准号2011189) |
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| 摘 要： | 研究了齐墩果酸在β-环糊精胶束体系中的荧光特性,发现β-环糊精对齐墩果酸的荧光有较强的增敏作用.据此建立了胶束增敏荧光光谱法测定齐墩果酸的新方法,其线性范围为1.0×10-5～4.0×10-4 mol/L,样品的平均回收率为93.80％～106.3％,根据IUPAC规定测得检出限为6.17×10-7 mol/L,表观摩尔吸光系数ε为1.623×106 L· mol-1·cm-1.并对包合机理进行了介绍,确定了实验中β-环糊精的最佳用量,考察了离子强度对包合体系的影响.
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| 关 键 词： | 齐墩果酸 荧光光谱 β-环糊精 |
Determination of Oleanolic Acid by Micellar Sensitized Fluorescence Spectrometry |
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| YAN Li-li
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BI Shu-yun
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PANG Bo
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WANG Yu.Determination of Oleanolic Acid by Micellar Sensitized Fluorescence Spectrometry[J].Acta Scientiarum Naturalium Universitatis Neimongol,2012,43(2):137-140. |
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| Authors: | YAN Li-li BI Shu-yun PANG Bo WANG Yu |
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| Institution: | (School of Chemistry,Changchun Normal University,Changchun 130032,China) |
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| Abstract: | The fluorescence characteristics of oleanolic acid(OA) in micellar system of β-cyclodextrin(β-CD)was studied.The experiment indicated that β-CD can greatly enhance the fluorescence of OA.Based on this result,a new method of micellar sensitized fluorescence spectrometry for the trace analysis of OA was developed.The linear range was 1.0×10-5~ 4.0×10-4 mol/L.The recovery of samples range from 93.80% to 106.3%.According to the IUPAC’s rules,a limit of detection was abtained.It was 6.17×10-7mol/L.Apparent molar absorption coefficient ε was 1.623×106 L·mol-1·cm-1.The inclusion mechanism of β-CD and oleanolic acid was studied.The optimum amount of β-CD was determined and the effect of ionic strength on OA-β-CD system was studied. |
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| Keywords: | oleanolic acid fluorescence spectrum β-cyclodextrin |
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