| 大鼠胎脑神经干细胞的分离和培养 |
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| 引用本文： | 李雪玲,扈廷茂,苏慧敏,陈明杰,于海泉.大鼠胎脑神经干细胞的分离和培养[J].内蒙古大学学报(自然科学版),2004,35(5):540-545. |
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| 作者姓名： | 李雪玲 扈廷茂 苏慧敏 陈明杰 于海泉 |
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| 作者单位： | 1. 内蒙古大学实验动物研究中心,哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特,010021
2. Monash Institute of Reproduction and Development,Clayton VIC 3168,Australia |
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| 基金项目： | 国家教育部重点项目(项目号:地方01019) |
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| 摘 要： | 胚龄14．5～16．5d(E14．5～16．5)的大鼠胎脑组织获得大鼠胎脑神经干细胞(rat fetal neural stem cells，rFNSCs)，培养于含有碱性成纤维细胞生长因子(basic fibroblast growth factor，bFGF)和表皮生长因子(epidermal growth factor，EGF)的无血清培养液DMEM／F12中，用^3H]胸苷掺入试验检测EGF和bFGF对大鼠胚胎神经干细胞分裂和增殖的影响．BrdU结合反应和nestin免疫组化检测显示培养细胞在早期时代约90％以上具有分裂增殖能力并显示nestln阳性，而且这些细胞在培养过程中可以分化神经元、星形胶质细胞和少突胶质细胞，证明分离培养的是神经干细胞，可用于移植、定向分化和基因转移的研究．
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| 关 键 词： | 大鼠胎儿神经干细胞 DMEM／F12 表皮生长因子 碱性成纤维细胞生长因子 |
| 文章编号： | 1000-1638(2004)05-0540-06 |
| 修稿时间： | 2003年9月1日 |
Isolation and Culture of Rat Fetal Neural Stem Cells |
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| LI Xue-ling,HU Ting-mao,SU Hui-min,CHEN Ming-jie,YU Hai-quan,John R. Morrison.Isolation and Culture of Rat Fetal Neural Stem Cells[J].Acta Scientiarum Naturalium Universitatis Neimongol,2004,35(5):540-545. |
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| Authors: | LI Xue-ling HU Ting-mao SU Hui-min CHEN Ming-jie YU Hai-quan John R Morrison |
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| Institution: | LI Xue-ling~1,HU Ting-mao~1,SU Hui-min~1,CHEN Ming-jie~1,YU Hai-quan~1,John R. Morrison~2 |
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| Abstract: | Rat fetal neural stem cells(rFNSC),removed from E14.5-16.5 rat fetal brain tissues, were cultured in serum-free medium DMEM/F12 contained basic fibroblast growth factor(bFGF) and epidermal growth factor(EGF).The inf1uences of bFGF and EGF on the proliferation of rat fetal neural stem cells were screened by ~3H] thymidine incorporation. The results from BrdU incorporation show that these cells have mitotic ability during culture, and above 90% of cells in culture are nestin positive.They can differentiate into neurons,oligodendrocytes and astrocytes.It is proved that the obtained cells are rat neural stem cells. |
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| Keywords: | rat fetal neural stem cells DMEM/F12 epidermal growth factor basic fibroblast growth factor |
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