| 农杆菌介导人肝再生增强因子转化河套蜜瓜的研究 |
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| 引用本文： | 刘明秋,张立全,格根通拉嘎,苗素平,扈廷茂.农杆菌介导人肝再生增强因子转化河套蜜瓜的研究[J].内蒙古大学学报(自然科学版),2006,37(6):656-659. |
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| 作者姓名： | 刘明秋 张立全 格根通拉嘎 苗素平 扈廷茂 |
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| 作者单位： | 内蒙古大学生命科学学院,呼和浩特,010021 |
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| 基金项目： | 内蒙古自然科学基金;内蒙古教育厅资助项目 |
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| 摘 要： | 利用三亲融合法将含有人肝再生增强因子(hum an augm en ter of liver regeneration,hALR)的果实特异性表达载体pPZP-CAN导入农杆菌LBA 4404,转化河套蜜瓜子叶外植体,经诱导与筛选获得了抗性再生植株,PCR扩增、PCR-Sou thern和斑点杂交检测证明hALR已整合入河套蜜瓜再生植株基因组中.
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| 关 键 词： | 人肝再生增强因子 河套蜜瓜 子叶 转基因 果实特异性 |
| 文章编号： | 1000-1638(2006)06-0656-04 |
| 收稿时间： | 2006-03-01 |
| 修稿时间： | 2006-05-19 |
Research of hALR Transformation in Cucumis melo L cv. Hetao by Agrobacterium tumefaciens |
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| LIU Ming-qiu,ZHANG Li-quan,Gegentonglaga,MIAO Su-ping,HU Ting-mao.Research of hALR Transformation in Cucumis melo L cv. Hetao by Agrobacterium tumefaciens[J].Acta Scientiarum Naturalium Universitatis Neimongol,2006,37(6):656-659. |
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| Authors: | LIU Ming-qiu ZHANG Li-quan Gegentonglaga MIAO Su-ping HU Ting-mao |
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| Institution: | College of Life Science, Inner Mongolia University, Hohhot 010021, China |
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| Abstract: | The fruit-specific expression vector pPZP-CAN,with human augmenter of liver regeneration gene,was introduced into Agrobacterium tumefaciens by triparental mating.Cucumis melo L.cv.Hetao cotyledons were transformed by Agrobacterium tumefaciens mediated procedure.The regenerated plants,which resistant to spectinomycin,were obtained.It was confirmed that human augmenter of liver regeneration gene was integrated into the genome of Cucumis melo L.cv.Hetao by the analysis of PCR,PCR-Southern and Dot blotting. |
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| Keywords: | hALR Cucumis melo L cv Hetao cotyledon transgenic fruit-specific |
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