南海圆鮀鲣肌肉组织酶解产物抗疲劳活性的初步研究

圆鮀鲣是我国南海高产低值的一类金枪鱼。对其肌肉组织的普通肉和暗色肉进行了酶解及氨基酸组成分析,并进行了抗疲劳活性动物实验评价。圆〖XCTUO-K.tif,+3.8mm。3.8mm;%110%110〗鲣的普通肉酶解产物冻干粉和暗色肉酶解产物冻干粉的氨基酸质量分数分别为57.06%和58.90%,其中支链氨基酸含量占总氨基酸的约18%。普通肉酶解产物冻干粉和暗色肉酶解产物冻干粉中分子质量5000Da以下肽的质量分数均达到70%以上,700Da以下的肽占20%以上。与空白对照组相比,圆鮀鲣的普通肉酶解产物冻干粉和暗色肉酶解产物冻干粉的各剂量组均具备显著延长小鼠负重游泳时间,增加小鼠肝糖原储备和降低运动后小鼠血清尿素氮含量的作用,表明2种酶解产物均有显著的抗疲劳活性,且二者无显著差异。研究结果表明,圆鮀鲣的普通肉和暗色肉组织均可作为抗疲劳保健产品潜在的开发对象。

Preliminary Study on Antifatigue Activity of Enzymatic Hydrolysate of Auxistapeionosoma Bleeker from South China Sea

Auxistapeionosoma Bleeker is a kind of tuna in the South China Sea with high yield and low value. This study analyzed the proximate composition, amino acid composition of the ordinary meat and dark meat hydrolysates, and evaluated their anti-fatigue activity by animal experiment. The total amino acids of the ordinary muscle hydrolysate powder (OMHP) and dark muscle hydrolysate powder (DMHP) of Auxistapeionosoma Bleeker were 57.06% and 58.90%, respectively. The branched chain amino acids accounted for about 18% of the total amino acids. The contents of the components in OMHP and DMHP with molecular weight below 5000Da were both up to 70%, and the content of below 700Da was both more than 20%.Compared with the control group, the OMHP and DMHP could significantly prolong the swimming time of mice (p<0.05), increase liver glycogen reserves of mice (p<0.05), and lower the serum urea nitrogen in mice after exercise (p<0.05). It showed that both had anti-fatigue activity, and there was no significant differences between them. The results showed that the OMHP and DMHP of Auxistapeionosoma Bleeker from South China Sea could be used as potential anti-fatigue health care products.