双水相萃取韭籽粕多糖的工艺优化及其抗氧化活性研究

为研究双水相萃取法提取韭籽粕多糖的效果,采用聚乙二醇-硫酸铵双水相体系提取韭籽粕多糖并探讨韭籽粕多糖的抗氧化活性。通过单因素实验和Design Expert 8.06响应面试验优化萃取工艺条件,得到优化工艺参数为聚乙二醇相对分子质量6000,聚乙二醇质量分数14.50%,硫酸铵质量分数20.46%,在此条件下多糖的萃取率为85.39%。在优化工艺条件下,测定韭籽粕多糖对DPPH·、·OH的清除能力以及总还原力,结果表明:韭籽粕多糖具有体外抗氧化活性,随着韭籽粕多糖质量浓度的提高,抗氧化活性增强。

Study on Optimal Technology and Antioxidant Activity of Polysaccharide Extracted by Two Aqueous Phase from Chinese Leek Seed Meal

To explore the effect of polysaccharide extraction by two-aqueous phase from Chinese leek seed meal, two aqueous phase of polyethylene glycol-ammonium sulfate extraction system was used to extract polysaccharide and its antioxidative activity was detected. The optimal conditions were obtained using single factor experiments and response surface methodology by Design Expert 8.06. The results indicated that the optimum extraction conditions were as follows:relative molecular mass of PEG 6000, mass fraction of PEG 14.50%, mass fraction of ammonium sulfate 20.46%. Under the optimal conditions, the extraction rate of polysaccharides from Chinese leek seed meal was 85.39%. The scavenging capacity of the polysaccharides to DPPH· and ·OH and its total reducing power was measured. The results showed that the polysaccharides from Chinese leek seed meal possessed antioxidant properties in vitro. And the antioxidant activity was enhanced gradually with the increasing concentration of polysaccharides.