| 酸性木聚糖酶交联酶聚集体的制备条件优化 |
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| 引用本文： | 吴春玲,李璠,马超,袁月,范光森,李秀婷.酸性木聚糖酶交联酶聚集体的制备条件优化[J].北京工商大学学报(自然科学版),2016,34(5):48-54. |
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| 作者姓名： | 吴春玲 李璠 马超 袁月 范光森 李秀婷 |
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| 作者单位： | 北京工商大学 食品学院, 北京 100048,北京工商大学 食品学院, 北京 100048,北京工商大学 食品学院, 北京 100048,北京工商大学 食品学院, 北京 100048,北京工商大学 食品学院, 北京 100048;北京市食品风味化学重点实验室, 北京 100048;北京食品营养与人类健康高精尖创新中心, 北京 100048,北京工商大学 食品学院, 北京 100048;北京市食品风味化学重点实验室, 北京 100048;北京食品营养与人类健康高精尖创新中心, 北京 100048 |
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| 基金项目： | 国家自然科学基金面上项目(31371723)；北京市自然科学基金青年项目(6164029)。 |
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| 摘 要： | 交联酶聚集体法是一种新型的无载体酶固定化方法,为提高酸性木聚糖酶的稳定性,使用该法固定化微紫青霉产酸性木聚糖酶,制备无载体固定化木聚糖酶,并对其制备条件进行优化。结果表明,优选的制备条件为将质量浓度0.36mg/mL的酸性木聚糖酶粗酶液在冰水浴中经饱和质量分数为85%的硫酸铵沉淀30min后,于40℃,加入终体积分数为0.14%的戊二醛,交联4h可获得较高活性的交联酶聚集体,酶活保留率达42.2%。这有助于酸性木聚糖酶更好地在工业中应用。
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| 关 键 词： | 微紫青霉 酸性木聚糖酶 交联酶聚集体 无载体固定化 |
| 收稿时间： | 2016/6/13 0:00:00 |
Optimizing Preparation Conditions of Cross-linked Acid-stable Xylanase Aggregates |
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| WU Chunling,LI Fan,MA Chao,YUAN Yue,FAN Guangsen and LI Xiuting.Optimizing Preparation Conditions of Cross-linked Acid-stable Xylanase Aggregates[J].Journal of Beijing Technology and Business University:Natural Science Edition,2016,34(5):48-54. |
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| Authors: | WU Chunling LI Fan MA Chao YUAN Yue FAN Guangsen and LI Xiuting |
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| Institution: | School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China,School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China,School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China,School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China,School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China;Beijing Key Laboratory of Flavor Chemistry, Beijing 100048, China;Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 100048, China and School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China;Beijing Key Laboratory of Flavor Chemistry, Beijing 100048, China;Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 100048, China |
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| Abstract: | |
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| Keywords: | Penicillium janthinellum acid-stable xylanase cross-linked enzyme aggregates carrier-free immobilized |
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