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蓝莓多酚的萃取及抗氧化活性研究
引用本文:程安玮,房凯,王文亮,刘丽娜,靳琼.蓝莓多酚的萃取及抗氧化活性研究[J].北京工商大学学报(自然科学版),2012,30(4):37-40.
作者姓名:程安玮  房凯  王文亮  刘丽娜  靳琼
作者单位:山东省农业科学院农产品研究所,山东济南,250100
基金项目:国家自然科学基金青年基金资助项目
摘    要:研究了不同萃取溶剂及pH值对蓝莓中可萃取多酚(EPP)和不可萃取多酚(NEPP)萃取率的影响,并用DPPH法研究了这两类多酚的抗氧化活性.结果表明,加入甲醇、乙醇、丙酮等有机溶剂可明显提高EPP的萃取率,其中以50%乙醇的萃取率最高.采用硫酸水解法分离制备NEPP,结果表明,蓝莓的NEPP含量远远高于EPP.EPP的抗氧化活性与其含量呈正相关性.萃取溶液pH值对EPP萃取率具有明显的影响,pH值越低,可萃取率越高.

关 键 词:蓝莓  可萃取多酚  不可萃取多酚  抗氧化活性

Extraction and Antioxidant Activity of Polyphenols in Blueberries
CHENG An-wei,FANG Kai,WANG Wen-liang,LIU Li-na and JIN Qiong.Extraction and Antioxidant Activity of Polyphenols in Blueberries[J].Journal of Beijing Technology and Business University:Natural Science Edition,2012,30(4):37-40.
Authors:CHENG An-wei  FANG Kai  WANG Wen-liang  LIU Li-na and JIN Qiong
Institution:(Institute of Agro-Food Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,China)
Abstract:The effect of solvent and pH on the contents of extractable polyphenols(EPP) and non-extractable polyphenols(NEPP) of blueberries was investigated in this paper.The antioxidant activity of polyphenols was also determined by DPPH system.The results showed that the solvent containing methanol,ethanol and acetone can enhance the EPP contents,especially 50% ethanol.The NEPP contents isolated by the hydrolysis of sulfuric acid were much higher than that of EPP.There is a good correlation between the antioxidant activity and the EPP content.The yield of EPP increased with the decreasing of pH.
Keywords:blueberry  extractable polyphenols  non-extractable polyphenols  antioxidant activity
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