| 用酪氨酸酶生物传感器测水中酚类物质的研究 |
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| 引用本文： | 穆冬燕,崔莉凤.用酪氨酸酶生物传感器测水中酚类物质的研究[J].北京工商大学学报(自然科学版),2002,20(1):12-16. |
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| 作者姓名： | 穆冬燕 崔莉凤 |
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| 作者单位： | 北京工商大学,化工学院,北京,100037 |
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| 摘 要： | 研究了用麦芽糊精修饰的酪氨酸酶碳糊电极构成电流型生物传感器测水中酚类污染物质的方法 .在外加电压为 - 1 0 0 m V( vs.SCE) ,p H值为 5 .40的磷酸盐缓冲溶液中 ,在浓度为 2 .0×1 0 -7～ 1 .0× 1 0 -5mol/L的范围内电极与苯酚的浓度有良好的线性关系 ,检出下限为 1 .0× 1 0 -7mol/L ,响应时间为 2 min.此电极对其他酚类物质如 :邻苯二酚 ,对氯苯酚 ,邻甲酚等都有良好的响应 .利用此种电极可用来检测工业废水中的酚类物质的含量
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| 关 键 词： | 生物传感器 电流型酶电极 酪氨酸酶 麦芽糊精 |
| 文章编号： | 1671-1513(2002)01-0012-05 |
| 修稿时间： | 2001年10月16日 |
RESEARCH OF TYROSINASE BIOSENSOR FOR DETECTION OF PHENOLS |
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| MU Dong yan,CUI Li feng.RESEARCH OF TYROSINASE BIOSENSOR FOR DETECTION OF PHENOLS[J].Journal of Beijing Technology and Business University:Natural Science Edition,2002,20(1):12-16. |
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| Authors: | MU Dong yan CUI Li feng |
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| Abstract: | Discussed a method for measuring phenols with a maltodextrin modified amprometric biosensor based on tyrosinase carbon paste electrode. When the working potential was -100 mV (vs.SCE),pH was 5 40 in the phosphate buffer solution and the time for measurement was 2 min,the detection limit was 1 0×10 -7 mol/L and the linear range was 2 0×10 -7 ～5 0×10 -5 mol/L. This method was also suitable for detection of catechol, o cresol and para chlorophenol, etc. This electrode could be applied to monitor the concentration of phenols in industry wastewater. |
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| Keywords: | biosensor amprometric enzyme electrode tyrosinase maltodextrin |
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