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响应面法对Neutrase蛋白酶酶解燕麦麸的优化研究
引用本文:王昌涛,冯冰,董银卯.响应面法对Neutrase蛋白酶酶解燕麦麸的优化研究[J].北京工商大学学报(自然科学版),2008,26(5).
作者姓名:王昌涛  冯冰  董银卯
作者单位:北京工商大学北京市植物资源研究开发重点实验室,北京,100048
摘    要:以燕麦麸皮为原料,采用Neutrase中性蛋白酶对其进行水解,以蛋白水解度及氮溶指数为评价指标,在反应时间、加酶量、料液比等单因素实验的基础上,采用Box-Benhnken响应面分析法系统探讨了燕麦麸蛋白酶解的最优条件,当水料比为15∶1,加酶量为底物的5%,酶解反应时间为4 h,水解度可以达到12%,氮溶指数达到59%.

关 键 词:酶解  燕麦麸  水解度  氮溶指数  响应面分析法

OPTIMIZATION OF ENZYMATIC HYDROLYSIS OF OAT BRAN BY NEUTRASE PROTEASE RESPONSE SURFACE METHOD
WANG Chang-tao,FENG Bing,DONG Yin-mao.OPTIMIZATION OF ENZYMATIC HYDROLYSIS OF OAT BRAN BY NEUTRASE PROTEASE RESPONSE SURFACE METHOD[J].Journal of Beijing Technology and Business University:Natural Science Edition,2008,26(5).
Authors:WANG Chang-tao  FENG Bing  DONG Yin-mao
Institution:WANG Chang-tao,FENG Bing,DONG Yin-mao(Beijing Key Laboratory of Plant Resources Research , Development,Beijing Technology , Business University,Beijing 100048,China)
Abstract:oat bran was taken as raw materials,which was hydrolyzed by neutral protease Neutrase.Based on single factor test including the reaction time,enzyme dosage and the ratio of material to water,hydrolysis degree and nitrogen solvuble index as evaluation index,the optimum process parameters of oat bran enzymatic hydrolysis were determined with Box-Benhnken response surface methodology,the optimum process parameters were the ratio of water to material was 15∶1,enzyme dosage was 5%,reaction time was 4 h,in this condition hydrolysis degree achieve 12%,nitrogen solvuble index achieve 59%.
Keywords:enzymatic hydrolysis  oat bran  hydrolysis degree  nitrogen solvuble index  response surface methodology  
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