| 好氧反硝化菌的物理诱变法育种研究 |
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| 引用本文： | 李永智,汪苹,王磊.好氧反硝化菌的物理诱变法育种研究[J].北京工商大学学报(自然科学版),2009,27(3). |
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| 作者姓名： | 李永智 汪苹 王磊 |
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| 作者单位： | 北京工商大学,化学与环境工程学院,北京,100048 |
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| 摘 要： | 以实验室SBR反应器分离出的好氧反硝化菌(WXZ-9)为原始菌株,分别用微波和紫外线诱变.通过反硝化性能测定筛选得到的正突变体WXZ-9-8号,96 h后的TIN去除率达到67.20%,比出发菌株WXZ-9号菌提高了7.07%,NO3--N去除率在24 h达到100%;WXZ-9-8号突变体的最大亚硝基氮还原速率达到52.89 mgNO2--N.L-1.d-1,比WXZ-9号菌株高6.90mgNO2--N.L-1.d-1.结果表明:WXZ-9-8号突变体具有更好的亚硝基氮还原酶的活性,并且比出发菌株有更好的生长适应性.
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| 关 键 词： | 好氧反硝化菌 微波诱变 紫外线诱变 |
STUDY ON MUTATION BREEDING WITH PHYSICAL METHODS BY AEROBIC DENITRIFIERS |
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| LI Yong-Zhi,WANG Ping,WANG Lei.STUDY ON MUTATION BREEDING WITH PHYSICAL METHODS BY AEROBIC DENITRIFIERS[J].Journal of Beijing Technology and Business University:Natural Science Edition,2009,27(3). |
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| Authors: | LI Yong-Zhi WANG Ping WANG Lei |
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| Institution: | College of Chemical and Environmental Engineering;Beijing technology and Business University;Beijing 100048;China |
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| Abstract: | Select aerobic denitrification strain(WXZ-9) isolated from SBR reactor in the laboratory as the original strain,mutate it with UV and microwave as the mutagens.The WXZ-9-8 Screening from mutants through mensured denitrification ability is the best,after 96 hours its reduction rate of TIN is 67.20%,it's 7.07% higher than the original strain,and the reduction rate of NO-3-N reach 100%;The maximum diminishing speed of WXZ-9-8 reach 52.89 mgNO-2-N·L-1·d-1,6.90 mgNO-2-N·L-1·d-1 higher than WXZ-9,so WXZ-9-8 has better activity of nitrite reductase,WXZ-9-8 has better adaptive growth too. |
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| Keywords: | aerobic denitrifiers microwave mutation UV-ray mutation |
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