| 米曲霉利用农业废弃物产木聚糖酶的条件优化 |
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| 引用本文： | 李勇如,李秀婷,孙宝国,宋焕禄,吕跃钢,宋红霞.米曲霉利用农业废弃物产木聚糖酶的条件优化[J].北京工商大学学报(自然科学版),2010,28(5):18-23. |
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| 作者姓名： | 李勇如 李秀婷 孙宝国 宋焕禄 吕跃钢 宋红霞 |
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| 作者单位： | 北京工商大学,化学与环境工程学院/食品添加剂与配料北京市高校工程研究中心,北京,100048 |
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| 基金项目： | 国家自然科学基金资助项目(31071511); 北京市属高等学校人才强教计划资助项目(PHR20110872); 北京工商大学教改项目(jg095206) |
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| 摘 要： | 主要研究了米曲霉利用农业废弃物产木聚糖酶的情况.对实验室保藏菌株米曲霉M-9生长所必需的碳源、氮源、培养温度和培养基初始pH等条件进行了优化.实验表明,此菌株最适碳源是粒度小于0.08 mm的玉米芯(质量分数为4.0%),最佳复合氮源是质量分数为1.2%的酵母提取物加质量分数为0.8%的大豆蛋白胨,最适初始pH值为7.5,最适产酶温度为30℃,最佳转速为200 r/m in,添加表面活性剂吐温60对菌株产酶有促进作用.在最适培养条件下,培养5 d,米曲霉沪酿M-9木聚糖酶的产量高达795.93 U/mL,是优化前的2.2倍.
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| 关 键 词： | 米曲霉 木聚糖酶 发酵 农业废弃物 |
OPTIMIZATION OF XYLANASE PRODUCTION FROM ASPERGILLUS ORYZAE BY USING AGRICULTURAL WASTES IN SUBMERGED FERMENTATION |
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| LI Yong-ru,LI Xiu-ting,SUN Bao-guo,SONG Huan-lu,Lv Yue-gang and SONG Hong-xia.OPTIMIZATION OF XYLANASE PRODUCTION FROM ASPERGILLUS ORYZAE BY USING AGRICULTURAL WASTES IN SUBMERGED FERMENTATION[J].Journal of Beijing Technology and Business University:Natural Science Edition,2010,28(5):18-23. |
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| Authors: | LI Yong-ru LI Xiu-ting SUN Bao-guo SONG Huan-lu Lv Yue-gang and SONG Hong-xia |
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| Institution: | LI Yong-ru,LI Xiu-ting,SUN Bao-guo,SONG Huan-lu,L(U) Yue-gang,SONG Hong-xia |
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| Abstract: | Cultural conditions for xylanase production by the strain Aspergillus oryzae M-9 were optimized under submerged fermentation.The strain secretes high levels of xylanase on the agricultural residues of Corn cobs as well as on xylans.The results of single-factor experiment showed that 0.08mm Corn cobs(4.0%) was the best carbon source and inducer.A combination of yeast extract(1.2%) and soybean peptone(0.8%) was the best nitrogen source.The optimal initial pH value,cultivation temperature and the rotation rate... |
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| Keywords: | Aspergillus oryzae xylanase fermentation agricultural wastes |
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