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复合酶法水解花生粕制备抗氧化肽的工艺优化
引用本文:刘红梅,师广波,李向东,孙中涛.复合酶法水解花生粕制备抗氧化肽的工艺优化[J].北京工商大学学报(自然科学版),2014,32(3):59-64.
作者姓名:刘红梅  师广波  李向东  孙中涛
作者单位:[1]山东农业大学生命科学学院,山东泰安271018 [2]山东农业大学农学院,山东泰安271018
摘    要:以DPPH自由基清除率和收率为响应值,采用响应面法对复合酶法水解花生粕生产抗氧化性肽工艺条件进行了优化.研究结果表明,碱性蛋白酶和中性蛋白酶对花生粕具有较强的水解能力,二者以2∶1的比例对花生粕水解时,较优水解条件是50℃,pH值8.54,底物质量分数8.34%.在此条件下酶解16 h,花生肽收率为65.80%,在花生肽质量浓度为0.55 mg/mL时,对DPPH自由基清除率为25.77%,与优化前相比,复合酶比碱性蛋白酶单酶水解时收率和DPPH自由基清除率分别提高了7.48%和7.66%,比中性蛋白酶单酶水解时分别提高了10.29%和22.53%.

关 键 词:碱性蛋白酶  中性蛋白酶  花生肽  抗氧化性
收稿时间:2013/10/16 0:00:00

Optimization of Processing of Antioxidant Peptides from Peanut Meal using Compound Enzymes
LIU Hongmei,SHI Guangbo,LI Xiangdong and SUN Zhongtao.Optimization of Processing of Antioxidant Peptides from Peanut Meal using Compound Enzymes[J].Journal of Beijing Technology and Business University:Natural Science Edition,2014,32(3):59-64.
Authors:LIU Hongmei  SHI Guangbo  LI Xiangdong and SUN Zhongtao
Institution:1. College of Life Science, Shandong Agricultural University, Tai' an 271018, China ; 2. College of Agronomy, Shandong Agricultural University, Tai ' an 271018, China)
Abstract:With the DPPH radical scavenging ability and yield of peanut peptides as response values, the Box-Behnken experiments were carried out to optimize the production conditions of antioxidant peptides from peanut meal using compound enzymes. Results proved that alealase and neutrase showed the highest hydrolysis ability compared with other commercial proteases. The optimal hydrolysis conditions were 50 ℃ , pH 8.54, and substrate concentration 8.34% when alcalase and neutrase were adopted together with a ratio of 2 to 1. When peanut meal was hydrolyzed for 16 h under the optimal conditions, the yield of peanut peptides reached 65.80% and the DPPH radical scavenging ability of the peanut peptides solution with a concentration of 0.55 mg/mL reached 25.77% , The yield and DPPH radical scavenging ability of peanut peptides obtained using compound enzymes were 7.48% and 7.66% higher than these of peptides obtained using alealase and 10. 29% and 22.53% higher than these of peptides obtained using neutrase.
Keywords:alcalase  neutrase  peanut peptides  antioxidant activity
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