| 高效液相色谱法测定镇江香醋中5-羟甲基糠醛的含量 |
| |
| 引用本文： | 张玉玉,宋弋,周婷婷,吕茜,杨瑞学,李全宏.高效液相色谱法测定镇江香醋中5-羟甲基糠醛的含量[J].北京工商大学学报(自然科学版),2012,30(2):39-42. |
| |
| 作者姓名： | 张玉玉 宋弋 周婷婷 吕茜 杨瑞学 李全宏 |
| |
| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083
2. 中国农业机械化科学研究院,北京,100083 |
| |
| 基金项目： | 国家“十二五”科技支撑计划项目 |
| |
| 摘 要： | 采用高效液相色谱法,对镇江香醋中5-羟甲基糠醛进行了定量分析.样品中的HMF含量的测定条件为:以甲醇-水溶液(体积比为5∶95)为流动相,流速为1.0 mL/min,采用Venusil XBP-C18色谱柱分离,柱温为30℃,UV检测器的检测波长为284 nm.定量分析结果表明,镇江香醋中HMF的含量为8.01 mg/kg.高效液相色谱法分析镇江香醋中HMF含量的方法测定准确,稳定性高,HMF在0.014 3～0.085 8μg的进样范围内线性相关系数为R2=0.999 9,平均回收率98.66%,相对标准偏差为5.53%.
|
| 关 键 词： | 镇江香醋 高效液相色谱 -羟甲基糠醛 含量检测 |
Determination of 5-hydroxymethylfurfural in
Zhenjiang Vinegar by HPLC |
| |
| ZHANG Yu-yu,SONG Yi,ZHOU Ting-ting,Lv Qian,YANG Rui-xue and LI Quan-hong.Determination of 5-hydroxymethylfurfural in
Zhenjiang Vinegar by HPLC[J].Journal of Beijing Technology and Business University:Natural Science Edition,2012,30(2):39-42. |
| |
| Authors: | ZHANG Yu-yu SONG Yi ZHOU Ting-ting Lv Qian YANG Rui-xue and LI Quan-hong |
| |
| Institution: | 1(1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China; 2.Chinese Academy of Agricultural Mechanization Science,Beijing 100083,China) |
| |
| Abstract: | A method for determination of 5-hydroxymethylfurfural by high-performance liquid chromatography in Zhenjiang vinegar was developed.Samples were analyzed on a Venusil XBP-C18 column with oven temperature of 30 ℃,using a mixture of methanol and water(5∶95,v/v) as the mobile phase with the flow rate of 1.0 mL/min,and detected at 284 nm.Within the linear range of 0.014 3～0.085 8 μg,the correlation coefficient was 0.999 9.The method was accurate with the average recoveries rate and relative standard deviation of 98.66% and 5.53%,respectively. |
| |
| Keywords: | Zhenjiang vinegar high-performance liquid chromatographic 5-hydroxymethylfurfural content determination |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《北京工商大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《北京工商大学学报(自然科学版)》下载免费的PDF全文 |
|
