| 响应面法优化微波辅助提取桑叶多糖的工艺研究 |
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| 引用本文： | 王尉,徐春明,曹学丽.响应面法优化微波辅助提取桑叶多糖的工艺研究[J].北京工商大学学报(自然科学版),2009,27(4):6-9. |
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| 作者姓名： | 王尉 徐春明 曹学丽 |
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| 作者单位： | 北京工商大学,北京市植物资源研究开发重点实验室,北京,100048 |
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| 摘 要： | 利用微波辅助技术进行桑叶多糖提取,通过单因素实验确定因素与水平,应用Box-Behnken设计3因素3水平的试验,依据回归分析确定最优的提取工艺条件.结果表明,微波辅助提取桑叶多糖的优化提取工艺条件为:温度88℃、时间11 min和液固比18∶1,提取的多糖含量为15.20 mg/g.微波辅助提取的多糖含量分别比传统水提法提取10 min和60 min高2.18倍和0.23倍.
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| 关 键 词： | 桑叶 多糖 微波辅助提取 响应面分析 |
OPTIMIZATION OF MICROWAVE-ASSISTED EXTRACTION OF POLYSACCHARIDE FROM MORUS ALBA L. BY RESPONSE SURFACE METHODOLOGY |
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| WANG Wei,XU Chun-ming,CAO Xue-li.OPTIMIZATION OF MICROWAVE-ASSISTED EXTRACTION OF POLYSACCHARIDE FROM MORUS ALBA L. BY RESPONSE SURFACE METHODOLOGY[J].Journal of Beijing Technology and Business University:Natural Science Edition,2009,27(4):6-9. |
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| Authors: | WANG Wei XU Chun-ming CAO Xue-li |
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| Institution: | Beijing Key Laboratory of Plant Resources Research and Development;Beijing Technology and Business University;Beijing 100048;China |
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| Abstract: | Microwave-assisted extraction was applied for polysaccharide extraction from Morus alba L..Factors and levels were optimized by single factor test.Box-Behnken design was used to 3-factors and 3-levels test.The optimal process conditions were obtained by regression analysis of response surface methodology.The results showed that the optimal polysaccharide extraction process conditions were obtained by microwave-assisted extraction as follows: extraction temperature was 88 ℃,extraction time was 11 minutes and liquid/solid ratio was 18∶1,the maximum of polysaccharide content was 15.20 mg/g.Polysaccharide content of microwave-assisted extraction was higher 2.18 fold(extraction time: 10 min) and 0.23 fold(extraction time: 60 min) than that of traditional extraction,respectively. |
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| Keywords: | Morus alba L polysaccharide microwave-assisted extraction response surface methodology |
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