| 量子点经嗅觉通道进入中枢神经系统的可视化过程 |
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| 引用本文： | 尹芳蕊,陈春英,董元兴,吴刚,高愈希.量子点经嗅觉通道进入中枢神经系统的可视化过程[J].科学通报,2010,55(7):547-552. |
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| 作者姓名： | 尹芳蕊 陈春英 董元兴 吴刚 高愈希 |
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| 作者单位： | 中国科学院高能物理研究所核分析技术重点实验室;纳米生物效应与安全性重点实验室;内蒙古包头医学院基础医学部;忻州师范学院物理系; |
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| 基金项目： | 国家自然科学基金(批准号:20777075);;国家重点基础研究发展计划(编号:2006CB705600);;中国科学院知识创新工程资助项目 |
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| 摘 要： | 模拟纳米颗粒呼吸道暴露及鼻腔给药模式, 给ICR雌性小鼠鼻腔滴注量子点溶液, 用动物活体荧光成像系统和荧光显微技术观察小鼠鼻腔、嗅球及大脑等组织中量子点分布随时间的变化情况. 动物活体荧光成像可见, 鼻腔滴注量子点30 min后, 量子点溶液扩散到嗅球, 2 h后鼻腔荧光强度逐渐变弱, 24 h仅在嗅球部位仍有可见荧光. 荧光显微镜观察组织切片可见, 滴注30 min时, 量子点仅存于嗅球前端边缘, 1 h后进入嗅球, 并向嗅球深层转移, 24 h后发现嗅球内量子点荧光变弱, 并在大脑组织切片内观察到量子点荧光. 结果表明, 纳米颗粒可以经过鼻黏膜进入脑组织, 分布于嗅球、大脑等部位.
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| 关 键 词： | 量子点 嗅觉通道 药物载体 活体荧光成像 荧光显微法 |
| 收稿时间： | 2009-05-31 |
A visible process of transporting quantum dots to the central nervous system through the olfactory pathway |
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| YIN FangRui,CHEN ChunYing,DONG YuanXing,WU Gang & GAO YuXi Key Laboratory of Nuclear Analytical Techniques , Key Laboratory for Biological Effects of Nanomaterials , Nanosafety.A visible process of transporting quantum dots to the central nervous system through the olfactory pathway[J].Chinese Science Bulletin,2010,55(7):547-552. |
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| Authors: | YIN FangRui CHEN ChunYing DONG YuanXing WU Gang & GAO YuXi Key Laboratory of Nuclear Analytical Techniques Key Laboratory for Biological Effects of Nanomaterials Nanosafety |
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| Institution: | YIN FangRui1,2,CHEN ChunYing1,DONG YuanXing3,WU Gang2 & GAO YuXi1 1 Key Laboratory of Nuclear Analytical Techniques , Key Laboratory for Biological Effects of Nanomaterials , Nanosafety,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100049,China,2 School of Basic Medical Sciences,Baotou Medical College,Baotou 014010,3 Department of Physics,Xinzhou Teachers University,Xinzhou 034000 |
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| Abstract: | To investigate the possibility of transportation of nanoparticles to the central nervous system directly through the olfactory pathway under respiratory exposure condition,ICR female mice were administered with the quantum dot solution using nasal drip simulating the respiratory exposure of nanoparticles.The process for the quantum dots passing through the olfactory pathway and entering into brain tissues was monitored with in vivo fluorescence imaging and fluorescent microscopy after slicing the tissues.In... |
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| Keywords: | quantum dots olfactory system drug carriers in vivo fluorescence imaging fluorescence microscopy |
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