光电化学免疫分析研究进展

光电化学分析是近年建立起来的一种基于物质的光电转换特性而进行检测的新型分析方法,它具有灵敏度高、设备简单、易于微型化等特点.光电化学免疫分析则是光电化学分析与免疫反应的结合,体现了光电化学传感的高灵敏度和免疫反应的高特异性.其基本方法原理是,在光照条件下,光电活性物质将免疫反应转变为电信号,从而实现对待测物的定量测定.在过去10年中,伴随着光电化学分析技术的进步,光电化学免疫分析经历了快速发展的过程.本文介绍了光电化学免疫分析的方法原理和特点,对其代表性的研究工作进行介绍和评述,并展望其未来的发展趋势.

Progress in photoelectrochemical immunoassay

Photoelectrochemical (PEC) analysis is a newly established analytical approach based on the photo-to-electrical properties of photoactive materials. It possesses many intrinsic advantages such as remarkable sensitivity, simple instrument and inherent miniaturization. On basis of the combination of PEC analysis and immunoassay, the PEC immunoassay has advantageously inherited the high sensitivity and specificity from the PEC analysis and the immunorecognition, respectively. Its general sensing mechanism is that, upon illumination, the photoactive materials could convert the specific immunoreactions into electrical signals, thereby realizing the quantification of various analytes. In past years, with the rapid development of PEC analysis, the PEC immunoassay has attracted considerable interests, and significant progresses have been achieved in its advancement and application. This review introduces the basic principles, classification, characteristics as well as the progress of PEC immunoassay. The future prospects in this area are also evaluated and discussed.