| L－谷氨酰胺生产菌定向选育的初步研究 |
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| 引用本文： | 刘永生,张凤珊,陈宁,李庆国,张克旭.L－谷氨酰胺生产菌定向选育的初步研究[J].天津科技大学学报,1994(1). |
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| 作者姓名： | 刘永生 张凤珊 陈宁 李庆国 张克旭 |
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| 作者单位： | 天津轻工业学院食品工程系 |
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| 摘 要： | 以天津短杆菌ＴＧ－８６６为出发菌株，利用亚硝基胍、硫酸二乙酯等常规诱变方法以及原生质体融合法最终筛选出一株谷氨酰胺生产菌ＧＭＮ－１８［ＭＳＯ~Ｒ＋８－ＡＧ~Ｒ＋ＳＧ~Ｒ＋（ＮＨ_４）_２ＳＯ_４~Ｒ］，在适宜条件下平均积累谷氨酰胺２７．３ｇ／Ｌ。
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| 关 键 词： | L－谷氨酰胺，原生质体融合，发酵 |
PRELIMINARY STUDIES ON THE BREEDING OF L-GLUTAMINE-PRODUCING STRAINS |
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| Liu Yongsheng, Zhang Fengshan, Chen Ning, Li Qingguo, Zhang Kexu.PRELIMINARY STUDIES ON THE BREEDING OF L-GLUTAMINE-PRODUCING STRAINS[J].Journal of Tianjin University of Science & Technology,1994(1). |
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| Authors: | Liu Yongsheng Zhang Fengshan Chen Ning Li Qingguo Zhang Kexu |
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| Institution: | Liu Yongsheng; Zhang Fengshan; Chen Ning; Li Qingguo; Zhang Kexu |
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| Abstract: | With Brevibacterium Tianjin TG-866 as original strain, an L-Glutamine producing mutant GMN-18 MSOR + 8-AGR + SGR + (NH4)2SO4R] was successfully obtained by using NTG and DES, and subsequent protoplast fusion. At optimum conditions.the mutant strain accumulates an average of 27.3g/L of glutamine. |
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| Keywords: | L-Glutamine Protoplast fusion Fermentation |
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