| 小鼠白细胞介素-18的克隆及真核表达质粒的构建 |
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| 引用本文： | 陈勇,赵雪花,何丽娅,成彩莲,夏瑞明,朱汉荣,王海燕. 小鼠白细胞介素-18的克隆及真核表达质粒的构建[J]. 武汉科技大学学报(自然科学版), 2006, 29(2): 200-202 |
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| 作者姓名： | 陈勇 赵雪花 何丽娅 成彩莲 夏瑞明 朱汉荣 王海燕 |
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| 作者单位： | 武汉科技大学医学院,湖北,武汉,430080 |
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| 摘 要： | 克隆小鼠白细胞介素-18（mIL-18）,并与真核表达质粒pcDNA3.1/HisB重组,构建真核表达重组质粒.采用RT-PCR,从小鼠肝细胞中扩增IL-18的全长cDNA.经Hind Ⅲ,EcoR Ⅰ双酶切,将该cDNA片断插入表达载体pcDNA3.1/HisB.通过酶切、PCR及测序对重组体进行鉴定.经鉴定,重组质粒构建正确,成功构建了真核表达载体pcDNA3.1/mIL-18,为下一步进行IL-18的功能研究奠定了基础.
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| 关 键 词： | 白细胞介素-18 真核表达质粒 |
| 文章编号： | 1672-3090(2006)02-0200-03 |
| 收稿时间： | 2005-06-17 |
| 修稿时间： | 2005-06-17 |
Cloning of Mouse Interleukin-18 and Construction of Eukaryotic Expression Plasmid |
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| CHEN Yong,ZHAO Xue-hua,HE Li-ya,CHENG Cai-lian,XIA Rui-ming,ZHU Han-rong,WANG Hai-yan. Cloning of Mouse Interleukin-18 and Construction of Eukaryotic Expression Plasmid[J]. Journal of Wuhan University of Science and Technology(Natural Science Edition), 2006, 29(2): 200-202 |
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| Authors: | CHEN Yong ZHAO Xue-hua HE Li-ya CHENG Cai-lian XIA Rui-ming ZHU Han-rong WANG Hai-yan |
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| Affiliation: | Medical College, Wuhan University of Science and Technology, Wuhan 430080, China |
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| Abstract: | The cDNA of mouse interleukin-18(IL-18) was cloned and recombined with plasmid pcDNA3.1/His B to construct an eukaryotic expression plasmid.The cDNA of mouse IL-18 was amplified from the liver cells by RTPCR.Having being digested by Hind III and EcoR I,this cDNA fragment was inserted into expression vector pcDNA3.1/His B.The recombinant plasmid was identified by restriction enzyme digest,PCR and sequencing.The identification has confirmed that the eukaryotic expression plasmid pcDNA3.1/ mIL-18 had been successfully constructed,which has provided foundation for further research. |
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| Keywords: | RT-PCR |
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