Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents. |
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Authors: | C Kleuss J Hescheler C Ewel W Rosenthal G Schultz B Wittig |
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Institution: | Institut für Molekularbiologie und Biochemie, Freie Universit?t Berlin, Germany. |
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Abstract: | The inhibition of voltage-dependent Ca2+ channels in secretory cells by plasma membrane receptors is mediated by pertussis toxin-sensitive G proteins. Multiple forms of G proteins have been described, differing principally in their alpha subunits, but it has not been possible to establish which G-protein subtype mediates inhibition by a specific receptor. By intranuclear injection of antisense oligonucleotides into rat pituitary GH3 cells, the essential role of the Go-type G proteins in Ca(2+)-channel inhibition is established: the subtypes Go1 and Go2 mediate inhibition through the muscarinic and somatostatin receptors, respectively. |
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