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| 烟草的组织培养技术研究 | |
| 引用本文： | 肖军,张云霄,刘伯峰. 烟草的组织培养技术研究[J]. 泰山学院学报, 2009, 31(6): 94-98 |
| 作者姓名： | 肖军 张云霄 刘伯峰 |
| 作者单位： | 1. 泰山学院生物科学与技术系,山东,泰安,271021 2. 清华大学生物系,北京,100084 |
| 摘 要： | 以烟草（Nicotiana tabacum L.）幼嫩叶片、茎段和带有叶片的茎段为外植体,对其进行组织培养研究.结果表明：烟草组织在MS＋0.1mg·L^-16-BA＋0.5mg·L^-1NAA的培养基中能诱导形成不定芽和根;在MS＋0.5mg·L^-16-BA＋0.1mg·L^-1NAA的培养基和MS＋0.5 mg·L^-16-BA＋0.5 mg·L^-1NAA的培养基中能脱分化形成愈伤组织,但只能诱导形成不定芽;带有叶片的茎段最容易诱导形成愈伤组织. |
| 关 键 词： | 烟草 组织培养 外植体 |
Study on Tissue Culture Technique of Nicotiana Tabacum |
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| XIAO Jun,ZHANG Yun-xiao,LIU Bo-feng. Study on Tissue Culture Technique of Nicotiana Tabacum[J]. Journal of Taishan University, 2009, 31(6): 94-98 | |
| Authors: | XIAO Jun ZHANG Yun-xiao LIU Bo-feng |
| Affiliation: | XIAO Jun ,ZHANG Yun - xiao, LIU Bo - feng ( 1. Department of Biological Science and Teehnology,Taishan University,Tai＇an,271021 ; 2. Department of Biology,Tsinghua University, Beijing, 100084, China) |
| Abstract: | Tissue culture of nicotiana tabacum was conducted by young leaves,stem segments and stem segments with leaves.The result showed that： The adventitious buds and roots were induced wihh culture of MS＋0.1mg·L^-1-BA＋0.5mg·L^-1AA;The callus were induced with culture of MS＋0.5mg·L^-1-BA＋0.1mg·L^-1AA and MS＋0.5mg·L^-1-BA＋0.5mg·L^-1AA,but only the adventitious buds were induced.The results also showed that the callus were induced the most easily on stem segments with leaves. |
| Keywords: | nicotiana tabacum tissue culture explant |
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