| 利用叶圆片法获得CMV_（CP）转基因烟草植株 |
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| 引用本文： | 张宗江,周钟信,刘艳军,尤明.利用叶圆片法获得CMV_（CP）转基因烟草植株[J].天津理工大学学报,1994(3). |
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| 作者姓名： | 张宗江 周钟信 刘艳军 尤明 |
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| 作者单位： | 天津农学院园艺系生物工程实验室，天津６７中 |
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| 摘 要： | 利用叶圆片法得到ＣＭＶＣＰ转基因烟草植株ＤＮＡ，ＲＮＡ与ＣＭＶＣＰ放射性标记探针点杂交及Ｓｏｕｔｈｅｒｎ印迹杂交的结果表明：ＣＭＶＣＰ基固可整合到烟草转基固株并且表达．叶面病毒接种后，转基因植株对ＣＭＶ病毒具有高抗性．
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| 关 键 词： | CMVCP，基因转化，烟草 |
OBTAINMENT OF CMVCP TRANSGENE-TOBACCO-PIANTS BY MEANS OF IEAF DISC MEATHODS |
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| Zhang Zongjiang, Zhou Zhongxin, liu Yianjin, You Ming.OBTAINMENT OF CMVCP TRANSGENE-TOBACCO-PIANTS BY MEANS OF IEAF DISC MEATHODS[J].Journal of Tianjin University of Technology,1994(3). |
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| Authors: | Zhang Zongjiang Zhou Zhongxin liu Yianjin You Ming |
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| Institution: | Zhang Zongjiang; Zhou Zhongxin; liu Yianjin; You Ming |
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| Abstract: | By the leaf disc meathods a number of CMVCP transgene-tobacco-plants areobtained.The resuIts of DNA, RNA point hebridization and DNA southern blot with theradioactive CMVcp probe indicated that the CMVCP genes are cooperated into andexpressed in tobacco genome. After leaf infection with virus, the transgeneplants showedhigher resistance to CMV virus. |
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| Keywords: | CMVCP genetransfor tobacco |
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