高效凝胶过滤色谱法测定菊糖聚合度

菊糖属于天然大分子多糖，其生理活性与性质受聚合度的影响。以OHpak SB-802.5 HQ为色谱柱，进样量为20 μL，柱温为30 ℃，流动相为0.3 mol/L NaNO3溶液，流速为0.3 mL/min，通过GPC 软件拟合三次方程标准曲线，利用高效凝胶色谱方法检测了9个样品的分子量并计算了其聚合度。结果表明，9个样品的菊糖聚合度在10~29，同种原材料的不同批次和不同品种间聚合度均存在差异。该方法为分析样品中菊糖的结构性质及控制产品质量提供了参考依据。

Development of high performance gel chromatography for thedetermination of inulin polymerization

Inulin is a type of natural macromolecular polysaccharide and its physiological activity and processing characteristics are affected by the degree of polymerization.In this study,OHpak SB-802.5 HQ was used as the chromatographic column.The injection volume was 20 μL,the column temperature was 30 ℃,the mobile phase was 0.3 moL/L NaNO₃ solution,and the flow rate was 0.3 mL/min.The cubic equation curve was fitted using GPC software.The molecular weights of nine samples were measured using high-performance gel chromatography and the degree of polymerization was calculated. Results showed that the inulin polymerization degree of inulin of nine samples ranged between 10 and 29 and the polymerization degree of different batches and varieties of the same raw material were different. The proposed method provides a reference for analyzing the structural properties and controlling the product quality of inulin.