| In situ localization of proteinase inhibitor mRNA in rice plant challenged by brown planthopper |
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| 引用本文： | WENGQingmei HUANGZhen WANGXiaolan ZHULili HEGuangcun. In situ localization of proteinase inhibitor mRNA in rice plant challenged by brown planthopper[J]. 科学通报(英文版), 2003, 48(10): 979-982. DOI: 10.1007/BF03184211 |
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| 作者姓名： | WENGQingmei HUANGZhen WANGXiaolan ZHULili HEGuangcun |
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| 作者单位： | KeyLaboratoryoftheMinistryofEducationforPlantDevelopmentalBiology,CollegeofLifeSciences,WuhanUniversity,Wuhan430072,China |
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| 摘 要： | Proteinase inhibitor (PI) mRNA was localized by in situ hybridization in tissue sections of root, stem and leaf of the resistant rice (B5) plant fed by brown planthopper nymphs. In the rice material without BPH feeding, PI gene was expressed in the root, stem and leaf, while the abundance of PI mRNA was low. In the rice material fed by BPH,PI gene was expressed substantially in the parenchyma of rice stem and leaf, but weakly in the root. The results indicated that the PI gene was up-regulated in the rice plant challenged by brown planthopper. For the first time, we reported the expression changes of proteinase inhibitor gene in plant which was infested by a piercing/sucking insect.
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| 关 键 词： | 原位定位 蛋白酶抑制剂 mRNA 水稻 褐飞虱 病虫害防冶 |
| 收稿时间： | 2003-03-03 |
In situ localization of proteinase inhibitor mRNA in rice plant challenged by brown planthopper |
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| Qingmei Weng,Zhen Huang,Xiaolan Wang,Lili Zhu,Guangcun He. In situ localization of proteinase inhibitor mRNA in rice plant challenged by brown planthopper[J]. Chinese science bulletin, 2003, 48(10): 979-982. DOI: 10.1007/BF03184211 |
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| Authors: | Qingmei Weng Zhen Huang Xiaolan Wang Lili Zhu Guangcun He |
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| Affiliation: | WENG Qingmei, HUANG Zhen, WANG Xiaolan, ZHU Lili & HE Guangcun Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China |
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| Abstract: | Proteinase inhibitor (PI) mRNA was localized by in situ hybridization in tissue sections of root, stem and leaf of the resistant rice (B5) plant fed by brown planthopper nymphs. In the rice material without BPH feeding, PI gene was expressed in the root, stem and leaf, while the abundance of PI mRNA was low. In the rice material fed by BPH, PI gene was expressed substantially in the parenchyma of rice stem and leaf, but weakly in the root. The results indicated that the PI gene was up-regulated in the rice plant challenged by brown planthopper. For the first time, we reported the expression changes of proteinase inhibitor gene in plant which was infested by a piercing/sucking insect. |
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| Keywords: | Bowman-Birk trypsin inhibitor in situ RNA hybridization brown planthopper plant defense response. |
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