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| 胆酸钠多克隆抗体的制备及其快速检测方法研究 | |
| 引用本文： | 陈江源,刘洁,蔡建中,付云洁,刘志国.胆酸钠多克隆抗体的制备及其快速检测方法研究[J].江汉大学学报(自然科学版),2012,40(2):77-81. |
| 作者姓名： | 陈江源 刘洁 蔡建中 付云洁 刘志国 |
| 作者单位： | 1. 江汉大学卫生技术学院,湖北武汉,430016 2. 江汉大学文理学院,湖北武汉,430056 3. 武汉工业学院生物与制药工程学院,湖北武汉,430023 |
| 摘 要： | 目的:探索用胶体金免疫层析法快速检测胆酸钠含量。方法:用碳二亚胺法合成的免疫抗原胆酸钠-BSA,免疫新西兰白兔,制备抗胆酸钠多克隆抗体。用酶联免疫吸附法(ELISA)鉴定特异性抗体效价。制备胆酸钠—OVA抗原—胶体金复合物,组装检测试纸。结果:胆酸钠抗体的效价为1∶80 000,建立的胶体金快速测定法对胆酸钠的检测限为12μg/mL。结论:试纸条法是快速检测胆汁酸含量的有效方式,在临床检测方面具有广阔的运用前景。 |
| 关 键 词： | 胆酸钠 多克隆抗体 ELISA 胶体金 试纸条 |
The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method |
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| CHEN Jiang-yuan,LIU Jie,CAI Jian-zhong,FU Yun-jie,LIU Zhi-guo.The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method[J].Journal of Jianghan University:Natural Sciences,2012,40(2):77-81. | |
| Authors: | CHEN Jiang-yuan LIU Jie CAI Jian-zhong FU Yun-jie LIU Zhi-guo |
| Institution: | 1.School of Health Science,Jianghan University,Wuhan,430016,Hubei,China; 2.College of Arts ＆ Science,Jianghan University,Wuhan,430056,Hubei,China; 3.School of Biotechnic and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,Hubei,China) |
| Abstract: | Objective：To develop colloidal gold immunochromatographic（IC） method for rapidly detecting the concentration of Sodium Cholate.Methods： Sodium Cholate-BSA immunogen was obtained via carbodiimide method.Then the immunoantigen was injected into New Zealand rabbits to prepare the anti-sodium cholate polyclona antibody according to routine immunity procedure.The specific antibody of Bile acid natrium was identified by indirect enzyme linked immunosorbent assay（ELISA）.Results： The sodium cholate-OVA antigen-colloidal gold compounds were made for test strip preparation.It was proved that the specificity of the antibody was 1 ∶ 80 000,and the detectability was 12 μg/mL by the method.Conclusion： Accordingly,IC test strip method is effective and sensitive for rapidly detecting sodium cholate,which will probably have a broad clinical application prospect. |
| Keywords: | sodium cholate polyclonal antibody ELISA colloidal gold test strip |
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