亲水性尿苷分子印迹聚合物的制备及分子选择性识别

研究亲水性核-壳结构分子印迹聚合物的制备并考察其吸附、分离性能.以尿苷为模板分子,通过回流沉淀聚合方法(DPP)制备亲水性的核-壳结构分子印迹聚合物微球.研究了模板分子UR与功能单体MMA之间的相互作用,考察了分子印迹聚合物对模板分子的吸附动力学、吸附平衡以及吸附选择性,评价了该分子印迹聚合物作为高效液相色谱柱填料时对尿苷及其类似物的分离能力.UR分子印迹聚合物对UR的吸附符合准一级动力学方程;Freund lich等温方程能更好地拟合分子印迹聚合物对印迹分子尿苷的等温吸附数据;UR分子印迹色谱柱能够实现UR及其类似物的分离,对尿苷具有选择性识别性能.

Synthesis and Selective Molecular Recognition of Hydrophilic Uridine Molecularly Imprinted Polymers

Hydrophilic molecularly imprinted core-shell structured polymers (MIPs) were prepared, and the adsorption and separation property of MIPs were investigated. Molecularly imprinted polymers (MIPs) were prepared using distillation precipitation polymerization (DPP) method. The interaction between template molecule UR and functional monomer MMA was studied by UV spectrometry. The adsorption kinetic behavior, equilibrium and selectivity of UR onto the MIPs were investigated by conducting a series of batch adsorption experiments. The separation capability of this MIPs to UR and its analog was evaluated when it worked as a HPLC column filling. The results show that the pseudo-first-order kinetic model provides a good correlation for the adsorption of UR onto MIPs. The adsorption of the imprinted molecule UR onto MIPs is fitted well to the Freundlich equation. The MIPs chromatographic column has a selective distinguish ability for UR and its analog.