| 淀粉酶高产菌株筛选及发酵条件优化研究 |
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| 引用本文： | 权淑静,马焕,解复红,谢宝恩,周伏忠.淀粉酶高产菌株筛选及发酵条件优化研究[J].河南科学,2011,29(10):1185-1189. |
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| 作者姓名： | 权淑静 马焕 解复红 谢宝恩 周伏忠 |
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| 作者单位： | 1. 河南省科学院生物研究所有限责任公司,郑州450008; 河南省微生物工程重点实验室,郑州 450008
2. 河南省科学院生物研究所有限责任公司,郑州,450008 |
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| 基金项目： | 河南省重大公益性科研项目(091100910500) |
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| 摘 要： | 从济源市麦田的土壤中筛选工业生产上有潜在应用价值的高产淀粉酶菌株,通过平板初筛和摇瓶复筛,得到高产淀粉酶细菌菌株P11 (2).对其产酶条件进行优化,最终得到最适碳源为可溶性淀粉,最适氮源为酵母粉和蛋白胨组合,加入MgSO4可以促进产酶,加入CaCl2或FeSO4抑制产酶.经正交试验确定培养基的最优组成,优化后的培养基...
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| 关 键 词： | 淀粉酶 筛选 优化 |
Screening of Amylase High Production Strains and Optimization of Production Conditions |
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| Quan Shujing,Ma Huan,Xie Fuhong,Xie Baoen,Zhou Fuzhong.Screening of Amylase High Production Strains and Optimization of Production Conditions[J].Henan Science,2011,29(10):1185-1189. |
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| Authors: | Quan Shujing Ma Huan Xie Fuhong Xie Baoen Zhou Fuzhong |
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| Institution: | Quan Shujing 1,2,Ma Huan 1,Xie Fuhong 1,Xie Baoen 1,Zhou Fuzhong 1 (1.Institute of Biology,Henan Academy of Sciences,Zhengzhou 450008,China,2.The Key Laboratory of Microbial Engineering of Henan Province,China) |
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| Abstract: | Amylases are important industrial enzymes with a wide range of applications.To obtain amylase with the potential application to food industry,the microorganisms from a sample of Jiyuan wheat field soil were screened and a bacteria strain,designated strain P11(2),producing high level of amylase was isolated.The enzyme producing medium was optimized by orthogonal tests.Soluble starch was found to be the good carbon source,and peptone plus yeast extract be the best nitrogen source.Enzyme production was stimula... |
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| Keywords: | amylase screening optimization |
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