| HIV-GAG质粒DNA疫苗的纯化工艺 |
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| 引用本文： | 刘新涛,吴丛梅,臧阳,孙博,殷玉和.HIV-GAG质粒DNA疫苗的纯化工艺[J].吉林大学学报(理学版),2013,51(4):720-723. |
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| 作者姓名： | 刘新涛 吴丛梅 臧阳 孙博 殷玉和 |
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| 作者单位： | 1. 长春工业大学 化学与生命科学学院, 长春 130012; 2. 吉林大学 生命科学学院, 长春 130012 |
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| 基金项目： | 国家科技重大项目(批准号:2012ZX10001-009-002) |
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| 摘 要： | 采用中空纤维超滤浓缩、 离子交换介质和复合模式凝胶过滤介质纯化获得了HIV-GAG药用级质粒DNA疫苗, 并对菌体培养、 菌体裂解、 粗提分离和柱层析等环节进行研究. 实验结果表明, 产品基本符合药用级的质粒DNA要求.
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| 关 键 词： | DNA疫苗 菌体裂解 离子交换介质 复合模式凝胶过滤介质 |
| 收稿时间： | 2012-10-22 |
HIV-DNA Vaccine Research of Purification Technology |
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| LIU Xin-tao,WU Cong-mei,ZANG Yang,SUN Bo,YIN Yu-he.HIV-DNA Vaccine Research of Purification Technology[J].Journal of Jilin University: Sci Ed,2013,51(4):720-723. |
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| Authors: | LIU Xin-tao WU Cong-mei ZANG Yang SUN Bo YIN Yu-he |
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| Institution: | 1. College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China;2. College of Life Science, Jilin University, Changchun 130012, China |
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| Abstract: | In order to get HIV GAG pharmaceutical grade plasmid DNA vaccine, we studied the procedure of the cultivation of bacteria, alkaline lysis, isolate rudely extraction and chromatography to get plasmid DNA. We used hollow fiber ultrafiltration concentration, ion exchange chromatography and composite model gel filtration medium in this study. The experimental results show that our final product meets the requirments of pharmaceutical standardization. And
this technology is suit for large scale production of DNA vaccines. |
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| Keywords: | DNA vaccine alkaline lysis ion exchange chromatography composite model gel filtration medium |
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