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胶体几丁质诱导红曲霉产几丁质酶的研究
引用本文:郑天柱,张庆庆,胡俊,谭胜,蒋汶.胶体几丁质诱导红曲霉产几丁质酶的研究[J].安徽工程科技学院学报,2014(4):1-3.
作者姓名:郑天柱  张庆庆  胡俊  谭胜  蒋汶
作者单位:安徽工程大学 微生物发酵安徽省工程技术研究中心,安徽 芜湖,241000
基金项目:国家大学生创新创业训练计划基金资助项目,芜湖市重点科技计划基金资助项目
摘    要:本文采用胶体几丁质为底物,研究了红曲霉液态发酵产几丁质酶.研究结果表明:胶体几丁质的添加能有效诱导红曲霉合成几丁质酶.在培养基中添加0.8%(W/V)胶体几丁质的同时,添加0.4%(W/V)葡萄糖,有利于红曲霉的生长以及几丁质酶的合成,发酵48h几丁质酶活力达到最高的0.3504U/mL.

关 键 词:液态发酵  红曲霉  胶体几丁质  几丁质酶

Induction effect of colloidal chitin to chitinase production by monascus
ZHENG Tian-zhu,ZHANG Qing-qing,HU Jun,TAN Sheng,JIANG Wen.Induction effect of colloidal chitin to chitinase production by monascus[J].Journal of Anhui University of Technology and Science,2014(4):1-3.
Authors:ZHENG Tian-zhu  ZHANG Qing-qing  HU Jun  TAN Sheng  JIANG Wen
Institution:(Coll. Of Bioch. Engn. ,Anhui Polytechnic University, Wuhu 241000, China)
Abstract:The study aimed to explore the chitinase production by Monascus liquid ferment-ation.The chitinase enzyme assay was carried out with colloidal chitin as a substrate.The experimental results indi-cate that addition of colloidal chitin helps the accumulation of chitinase.The highest activity of chitinase of 0.350 4 U/mL was obtained at 48 hours in themedium with the glucose 0.4%(W/V)and colloidal chitin 0.8%(W/V).
Keywords:liquid fermentation  monascus  colloidal chitin  chitinase
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