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| HEK293细胞中CDK10蛋白复合体的分离纯化 | |
| 引用本文： | 赵长宏,陈斌,吴传芳,欧阳劲,秦岭.HEK293细胞中CDK10蛋白复合体的分离纯化[J].四川大学学报(自然科学版),2013,50(1):187-192. |
| 作者姓名： | 赵长宏 陈斌 吴传芳 欧阳劲 秦岭 |
| 作者单位： | 1. 四川大学生命科学学院,成都,610064 2. 成都南诺格生物科技有限责任公司,成都,610064 3. 四川大学生命科学学院,成都610064;成都华高瑞甜科技有限公司,成都610064 |
| 基金项目： | 国家自然科学基金（30970625） |
| 摘 要： | |
| 关 键 词： | CDK10 蛋白纯化 细胞周期蛋白依赖性蛋白激酶 银染 |
| 收稿时间： | 2012/2/24 0:00:00 |
Isolation and Purification of the CDK10 protein complex from HEK293 cell |
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| ZHAO Chang-Hong , CHEN Bin , WU Chuan-Fang , OUYANG Jin , QIN Ling.Isolation and Purification of the CDK10 protein complex from HEK293 cell[J].Journal of Sichuan University (Natural Science Edition),2013,50(1):187-192. | |
| Authors: | ZHAO Chang-Hong CHEN Bin WU Chuan-Fang OUYANG Jin QIN Ling |
| Institution: | College of Life Sciences, Sichuan University;College of Life Sciences, Sichuan University;College of Life Sciences, Sichuan University;Chengdu Nanog Biological Technology Co., Ltd,;College of Life Sciences, Sichuan University;Chengdu Wagott Ruitian Technology Co., Ltd |
| Abstract: | The expression of Cdk10 was checked in HEK293 HeLa and MCF7 cells using Semi Quantitative RT PCR. Plasmid pcDNA3.1(-) Cdk10 Flag was constructed after cloning of Cdk10. HEK293 cell lines that can stably express Cdk10 Flag were cultured in large numbers. Cdk10 complex was purified from cell extractions using anti Flag immunoprecipitation under native conditions. The purified Cdk10 complex paved the way for future MS identification. |
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