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桔梗愈伤组织诱导及植株再生研究
引用本文:李玉芬,弭晓菊.桔梗愈伤组织诱导及植株再生研究[J].哈尔滨师范大学自然科学学报,1998,14(1):81-83.
作者姓名:李玉芬  弭晓菊
作者单位:哈尔滨工业大学(李玉芬),哈尔滨师范大学(弭晓菊)
摘    要:利用组织培养技术对桔梗不同外植体-根,茎叶进行了愈伤组织诱导植株再生过程研究,成功地诱导桔梗产生愈伤组织并获得了分化苗。外植体根,茎,叶愈伤组织诱导的频率不同。筛选出最佳的愈伤组织诱地的培养基及苗分化培养基。

关 键 词:桔梗  愈伤组织  植株再生  药用植物  育种

THE STUDY OF CALLUS INDUCE AND PLANT REGENERATION ON PLATYCODON GRANDIFLORUM A.DC.
Li,Yufen.THE STUDY OF CALLUS INDUCE AND PLANT REGENERATION ON PLATYCODON GRANDIFLORUM A.DC.[J].Natural Science Journal of Harbin Normal University,1998,14(1):81-83.
Authors:Li  Yufen
Institution:Li Yufen (Harbin Institute of Technology) Mi Xiaoju (Harbin Normal University)
Abstract:This paper studied the callus induce and plant regeneration of Platycodon grandiflorum A.DC. by applying technique of tissue culture.The callus was induced successfully and the seedings were obtained.The frequency of callus induce was different on root,stem and leaves.The suitable media were seclected for callus induce and differentiation of seeding. (1) The medium of callus induce is MS+6-BA1mg/L+NAA0.5mg/L pH5.6; (2) the medium of differentiation of seeding is MS+2.4-D0.2mg/L+6-BA1mg/L+NAA0.2mg/L pH5.6.
Keywords:Platycodon grandiflorum A  DC    Callus induce  Plant regeneration  
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