Differential protein expression in pancreatic islets after treatment with an imidazoline compound |
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| Authors: | T. Jägerbrink H. Lexander C. Palmberg J. Shafqat V. Sharoyko P.-O. Berggren S. Efendic S. Zaitsev H. Jörnvall |
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| Affiliation: | (1) Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden;(2) Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden;(3) The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Stockholm, Sweden;(4) Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia |
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| Abstract: | ![]()
The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. Whole cell extracts from isolated drug-treated and native pancreatic rat islets were compared after separation by 2-D gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry; 15 proteins were selectively up-regulated and 7 selectively down-regulated in drug-treated islets. Of special interest among the differentially expressed proteins are those involved in protein folding (Hsp60, protein disulfide isomerase, calreticulin), Ca2+ binding (calgizzarin, calcyclin and annexin I) and metabolism or signalling (pyruvate kinase, alpha enolase and protein kinase C inhibitor 1). Received 19 March 2007; received after revision 11 April 2007; accepted 11 April 2007 |
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| Keywords: | 2-D gel electrophoresis pancreatic islets proteomics type 2 diabetes imidazolines BL11282 |
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