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| 微孔板化学发光免疫分析法检测人血清中三碘甲状腺原氨酸 | |
| 引用本文： | 汪阿恋,肖勤,应希堂,陈国南,林金明.微孔板化学发光免疫分析法检测人血清中三碘甲状腺原氨酸[J].科学通报,2010,55(1):20-25. |
| 作者姓名： | 汪阿恋 肖勤 应希堂 陈国南 林金明 |
| 作者单位： | ① 福州大学化学化工学院, 福州 350002; ② 清华大学化学系, 北京 100084; ③ 北京科美东雅生物技术有限公司, 北京 100094 |
| 摘 要： | 建立了一种定量人血清中三碘甲状腺原氨酸(T3)的化学发光免疫分析方法, 使用辣根过氧化物酶(HRP)标记T3抗原, HRP催化鲁米诺-过氧化氢化学发光为检测体系. 对几种影响因素, 如包被抗体、酶标物的滴度进行选择, 对温育及检测时间等进行优化. 进行了方法学评价: 灵敏度为0.07 ng/mL; 批内和批间变异均小于10%; 回收率在80%~90%之间; 与结构相似物T4及rT3均无明显交叉. 使用本方法与现有的发光试剂盒进行比对, 相关性良好, 相关系数为0.9547. |
| 关 键 词： | 三碘甲状腺原氨酸 化学发光免疫分析 辣根过氧化物酶 |
| 收稿时间： | 2009-05-11 |
| 修稿时间： | 2009-09-27 |
Microplate chemiluminescence immunoassay for determination of triiodothyronine in human serum |
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| WANG ALian,XIAO Qin,YING XiTang,CHEN GuoNan,LIN JinMing College of Chemistry , Chemical Engineering,Fuzhou University,Fuzhou.Microplate chemiluminescence immunoassay for determination of triiodothyronine in human serum[J].Chinese Science Bulletin,2010,55(1):20-25. | |
| Authors: | WANG ALian XIAO Qin YING XiTang CHEN GuoNan LIN JinMing College of Chemistry Chemical Engineering Fuzhou University Fuzhou |
| Institution: | 1 College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002; 2 Department of Chemistry, Tsinghua University, Beijing 100084; 3 Beijing Chemclin Biotech Co., Ltd., Beijing 100094 |
| Abstract: | A microplate chemiluminescence immunoassay(CLIA) was developed for the clinical determination of Triiodothyronine(T3) in human serum,and the luminal-hydrogen peroxide was used as chemiluminescence system catalyzed by horseradish peroxidase(HRP).Several physicochemical parameters such as the dilution ratios of coated antibody and HRP-T3 conjugate was chose,the incubation time and detection time were optimized.Methodology evaluation was done:the detection limit was 0.07 ng/mL;intra-assay and inter-assay coeff... |
| Keywords: | triiodothyronine chemiluminescence immunoassay horseradish peroxidase |
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