| 鸭胚胎血液中PGCs数量及其外源DNA的体内转染 |
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| 引用本文： | 叶荣忠,张彦定,高建民,郭小玲.鸭胚胎血液中PGCs数量及其外源DNA的体内转染[J].厦门大学学报(自然科学版),2002,41(5):623-627. |
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| 作者姓名： | 叶荣忠 张彦定 高建民 郭小玲 |
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| 作者单位： | 1. 福建师范大学生物工程学院,福建,福州,350007
2. 厦门大学海洋与环境学院,福建,厦门,361005 |
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| 基金项目： | 国家自然科学基金 (39770 377)，福建省自然科学基金资助项目 |
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| 摘 要： | 抽取了麻鸭和骡鸭胚胎发育第14－20期的血液，制作血涂片，PAS染色，观察血液中的原生殖细胞的含量，并对各个时期血液中原生殖细胞的含量进行了统计，最高含量都出现在第16期,分别为8．9752％和2．1871％，用In vivo Gene Delivery Systern和pEGFP-N对PGCs进行了外源DNA的体内转染。结果在6只胚胎中的3只中发现GFP报告基因的表达。
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| 关 键 词： | 胚胎血液 PGCs 外源DNA 体内转染 麻鸭 骡鸭 原生殖细胞 胚胎发育 细胞数量 基因转移 |
| 文章编号： | 0438-0479(2002)05-0623-05 |
| 修稿时间： | 2002年5月20日 |
Observations of the Primordial Germ Cells in Embryonic Blood and in Vivo DNA Transfer into the Primordial Germ Cells in Sheldrake |
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| YE Rong-zhong ,ZHANG Yan-ding ,GAO Jian-min ,GUO Xiao-ling.Observations of the Primordial Germ Cells in Embryonic Blood and in Vivo DNA Transfer into the Primordial Germ Cells in Sheldrake[J].Journal of Xiamen University(Natural Science),2002,41(5):623-627. |
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| Authors: | YE Rong-zhong ZHANG Yan-ding GAO Jian-min GUO Xiao-ling |
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| Institution: | YE Rong-zhong 1,ZHANG Yan-ding 1,GAO Jian-min 1,GUO Xiao-ling 2 |
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| Abstract: | The amount of the primordial germ cells in embryonic blood from stages 14 through stages 20 in the Sheldrake's and mule-duck's embryos were counted by PAS reaction. The results showed that the highest concentration of primordial germ cells was at stages 16 both in Sheldrake and mule-duck, and the concentration was 8.975 2% for Sheldrake and 2.187 1% for mule-duck. Meanwhile, GFP report gene transfer into Sheldrake's blood PGCs in vivo was processed. The results showed that the expressions of GFP were found in 3 embryos. |
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| Keywords: | Sheldrake primordial germ cells DNA Transfer |
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