胎儿宫内发育迟缓疾病标志物的初步研究

目的筛选胎儿宫内发育迟缓大鼠血清中差异表达的蛋白质，寻找与胎儿宫内发育迟缓相关的疾病标志物。方法收集大鼠疾病正常组、IUGR组血浆各3例；TCA法沉淀血清蛋白，建立疾病组和正常组蛋白质的双向凝胶电泳图谱；ImageMaster分析软件分析差异蛋白质；飞行时间质谱（MALDI—TOF—MS）获得差异蛋白肽指纹图谱，MASCOT引擎搜索蛋白质种类。结果鉴定出个差异蛋白点3个，分别为：IGF—I、IGF-Ⅱ、IGFBP-3。结论IGF—I、IGF-II、IGFBP-3，可能是胎儿宫内发育迟缓疾病诊断的生物标志物。

Preliminary Study of Differential Proteins of Intrauterine Growth Retardation

Objective Differential serum proteins of rat with Intrauterine Growth Retardation were identifieated with proteomies teehnology ,and associated disease markers of intrauterine growth retardation were found. Method 3 cases rats plasma of normal group and IUGR group were collected, respectively. After serum proteins were extracted with TCA precipitation method ,We established proteins Image of two-dimensional gel electrophoresis of IUGR group and normal grotip. Differential proteins were screened with Image Master software, and peptide mass finger- printing were obtained by MALDI-TOF-MS, then dstabase were searched through the Mascot data engine, and protein identification results were obtained. Results 3 differential protein spots were identificatied. Conclusion IGF- I , IGF- I1,IGFBP-3 are possible bio-markers of In- trauterine Growth Retardation diagnosis.