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动物源乳酸菌的筛选与特性研究
引用本文:肖静,薛春梅,梁秀娟,刘晓兰,孙剑秋,吴耘红,李波.动物源乳酸菌的筛选与特性研究[J].高师理科学刊,2010,30(6):60-62.
作者姓名:肖静  薛春梅  梁秀娟  刘晓兰  孙剑秋  吴耘红  李波
作者单位:[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]佳木斯大学生命科学学院,黑龙江佳木斯154007 [3]齐齐哈尔市质量技术监督局,黑龙江齐齐哈尔161006
基金项目:黑龙江省科技厅技术研究项目,齐齐哈尔市科技局项目
摘    要:从健康的鸡、鼠、兔肠道内容物及胃内分离得到6株乳酸菌,通过单层平板扩散试验筛选出了3种有明显抑菌活性代谢产物的乳酸菌.用盐酸和过氧化氢对照实验,排除酸和过氧化物的干扰,用胰蛋白酶处理后抑菌活性明显降低,确定该乳酸菌能够发酵生产细菌素类抑菌物质.

关 键 词:乳酸菌  细菌素  抑菌实验

Study on isolation and characteristics of lactic acid bacteria from animals
XIAO Jing,XUE Chun-mei,LIANG Xiu-juan,LIU Xiao-lan,SUN Jian-qiu,WU Yun-hong,LI-Bo.Study on isolation and characteristics of lactic acid bacteria from animals[J].Journal of Science of Teachers'College and University,2010,30(6):60-62.
Authors:XIAO Jing  XUE Chun-mei  LIANG Xiu-juan  LIU Xiao-lan  SUN Jian-qiu  WU Yun-hong  LI-Bo
Institution:1.School of Life Science and Agroforestry,Qiqihar 161006,China;2.School of Life Science,Jiamusi University,Jiamusi 154007,China;3.Qiqihar Administration of Quality and Technology Supervision,Qiqihar 161006,China)
Abstract:Eleven strains of lactic acid bacteria were isolated from gastro-intestinal,and gastric of a health chickens,rats,rabbits.Through single-level slab proliferation experiment screening out three strains of lactic acid bacteria which has obvious bacteriostasis function.It is the product of the metabolism of lactic acid bacteria.Using HCl contrast experiments,exclude acid disturbance,activeness obviously reduced after trypsin processing,with catalase processing,the bacteriostasis effect was invariable,explained the catalase has not affected.Thus knew in the bacteriostasis matter has the bacteriocin of protein category.
Keywords:lactic acid bacteria  bacteriocin  bacteriostasis experiment
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