| L-抗坏血酸紫外分光光度法检测微量过氧化氢 |
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| 引用本文： | 张光学,王胤琪.L-抗坏血酸紫外分光光度法检测微量过氧化氢[J].咸宁学院学报,2008,28(6):99-101. |
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| 作者姓名： | 张光学 王胤琪 |
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| 作者单位： | 咸宁学院,化学与生命科学学院,湖北,咸宁,437100 |
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| 摘 要： | 建立了L-抗坏血酸（L—Aseorbieacid）紫外分光光度法测定微量过氧化氢的新方法．在pH=5．7的磷酸缓冲溶液介质中，选择273nm作为测定波长，过氧化氢的摩尔浓度在0～0．1mmol／L范围内服从比尔定律．回归方程为：y=0．0049＋4．44612x（mmol／L），检出限为0．401μmol／L．该法用于溶液中、生物组织等样品中微量过氧化氢浓度的测定，快速简便．
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| 关 键 词： | L-抗坏血酸 紫外分光光度法 微量 过氧化氢 氧化还原反应 |
Use L-ascorbic Acid UV-spectrophotometry to Determine the Trace Hydrogen Peroxide |
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| ZHANG Guang-xue,WANG Yin-qi.Use L-ascorbic Acid UV-spectrophotometry to Determine the Trace Hydrogen Peroxide[J].Journal of Xianning College,2008,28(6):99-101. |
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| Authors: | ZHANG Guang-xue WANG Yin-qi |
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| Institution: | (College of Chemistry and Life Science , Xianning University, Xianning 437100,China) |
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| Abstract: | A new method for determination of hydrogen peroxide in solution supply by UV - spectrophotometry was established. In a phosphate buffer medium of pH = 5.7 with an absorption wavelength 273nm, Beer,law is obeyed in the range of 0 - 0. l mmol/L for hydrogen peroxide and the linear regression equation is： y = 0. 0049 ＋ 4. 44612x（mmol/L） ;The detection limit of hydrogen peroxide is 0. 40lμmol/L. The method has been applied in rapid determination of hydrogen peroxide in some solution and organisms. |
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| Keywords: | L-ascorbic acid UV-spectrophotometry Hydrogen peroxide solution Redox reaction |
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