| 采用高效液相色谱法测定细菌纤维素合成培养基中的糖含量 |
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| 引用本文： | 颜金科,刘海芳,冯玉红,虞瑞雷,王荔宁,林强.采用高效液相色谱法测定细菌纤维素合成培养基中的糖含量[J].海南大学学报(自然科学版),2012,30(2):141-145. |
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| 作者姓名： | 颜金科 刘海芳 冯玉红 虞瑞雷 王荔宁 林强 |
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| 作者单位： | 1. 海南大学材料与化工学院,海南海口,570228
2. 海南师范大学,海南海口,571158 |
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| 基金项目： | 国家自然科学基金资助，海南大学科研项目，海南大学"211工程"项目 |
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| 摘 要： | 采用高效液相色谱（HPLC）法定量分析了细菌纤维素培养过程中椰子水培养基中的各糖含量变化,建立了椰子水培养基中N-乙酰-D-葡萄糖胺（GlcNAc）含量的检测方法.结果显示,该方法操作方便且分辨率高,在整个培养过程中,总糖含量急剧减小,其中葡萄糖消耗最快,果糖次之,但GlcNAc含量缓慢减少后其含量则逐渐增加.
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| 关 键 词： | N-乙酰-D-葡萄糖胺 高效液相色谱 木醋杆菌 椰子水 细菌纤维素 生物合成 |
Determination of Sugar Contents in the Synthetical Culture Medium of Bacterial Cellulose by High Performance Liquid Chromatography |
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| YAN Jin-ke
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LIU Hai-fang
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FENG Yu-hong
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YU Rui-lei
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WANG Li-ning
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LIN Qiang.Determination of Sugar Contents in the Synthetical Culture Medium of Bacterial Cellulose by High Performance Liquid Chromatography[J].Natural Science Journal of Hainan University,2012,30(2):141-145. |
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| Authors: | YAN Jin-ke LIU Hai-fang FENG Yu-hong YU Rui-lei WANG Li-ning LIN Qiang |
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| Institution: | 1.College of Materials and Chemical Engineering,Hainan University,Haikou 570228,China; 2.Hainan Normal University,Haikou 571158,China) |
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| Abstract: | In our report,high performance liquid chromatographic(HPLC) was used to determine the sugar content in the coconut-water culture medium and their amount changing during the culture process of bacterial cellulose,and the method by which N-acetylglucosamine(GlcNAc) was detected was set up.The results indicated that this method was easy to apply and was of high sensitivity.During the whole culture process,the total sugar content decreased rapidly,among which glucose consumption was the fastest,fructose consumption was lower than glucose,and GlcNAc levels decreased gradually after the slow increase. |
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| Keywords: | N-Acetyl-D-glucosamine high performance liquid chromatographic(HPLC) Acetobacter xylinum coconut-water bacterial cellulose biosynthesis |
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