| 农杆菌介导GNA基因对水稻的遗传转化 |
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| 引用本文： | 刘志学,何艺园,曹清玉,王卉,徐亚南,马向前,唐克轩.农杆菌介导GNA基因对水稻的遗传转化[J].复旦学报(自然科学版),2000,39(3):277-281. |
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| 作者姓名： | 刘志学 何艺园 曹清玉 王卉 徐亚南 马向前 唐克轩 |
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| 作者单位： | 1. 上海大学生命科学学院,上海 201800
2. 生命科学学院,遗传学研究所 |
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| 基金项目： | 国家科委八六三高科技资助项目 (10 1 0 10 10 1)，洛氏基金资助课题 |
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| 摘 要： | 在ｐＣＡＭＢＩＡ３３００质粒中插入带有ＲＳｓ－１启动子的ＧＮＡ基因，并利用农杆菌介导的遗传转化将其导入水稻的微不定芽受体，得到了再生植株。ＰＣＲ，Ｓｏｕｔｈｅｒｎ ｂｌｏｔ和Ｗｅｓｔｅｒｎ ｂｌｏｔ的检测结果初步证明ＧＮＡ基因已经整合到水稻的基因组中并得到表达。
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| 关 键 词： | 农杆菌 GNA基因 遗传转化 水稻 介导 |
| 文章编号： | 0427-7104(2000)03-0277-05 |
Agrobacterium tumefaciens-Mediated Transformation of Rice for GNA Gene |
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| LIU Zhi-xue,HE Yi-yuan,CAO Qing-yu,WANG Hui,XU Ya-nan,MA Xiang-qian,TANG Ke-xian.Agrobacterium tumefaciens-Mediated Transformation of Rice for GNA Gene[J].Journal of Fudan University(Natural Science),2000,39(3):277-281. |
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| Authors: | LIU Zhi-xue HE Yi-yuan CAO Qing-yu WANG Hui XU Ya-nan MA Xiang-qian TANG Ke-xian |
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| Institution: | LIU Zhi-xue
,HE Yi-yuan
,CAO Qing-yu
,WANG Hui
,XU Ya-nan
,MA Xiang-qian
(Institute of Genetics, School of life Scineces) |
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| Abstract: | GNA gene promoted by RSs 1 promoter was constructed into the pCAMBIA3300 plasmid vector, then transformed into micro adventitious bud of rice mediated by Agrobacterium tumefaciens . Regenerated plants were obtained from the transformed bud. The preliminary results of PCR, Southern blot and Western blot for the transgenic plants showed that GNA gene was introduced into the rice cells and expressed. |
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| Keywords: | Agrobacterium tumefaciens GNA gene Transformation Rice |
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