2株真菌修复石油污染土壤及对植物幼苗生长的影响

从石油污染的盐碱土壤中分离出具有降解石油性能的两株真菌,将其制成混合菌剂.通过掺混一定比例的石油污染原状土盆栽实验,以石油烃降解率、土壤脱氢酶活性和土壤的微生物多样性等为指标,测定了不同培养时间内各指标的变化.实验结果表明:受试土壤的总石油烃含量为21 000 mg/kg的情况下,加入5%菌剂后第56 d,真菌降解石油的效率达33.13%.在水培条件下,设置两类处理,即单独种植植物(分别为紫花苜蓿、燕麦)、微生物分别与两种植物组合,以表征植物根系活力的根脱氢酶活性和表征叶片脂质过氧化的丙二醛(MDA)含量为指标,研究了真菌促进植物的生长的作用.结果表明:添加5%菌剂在一定程度上提高了植物的根系活力,明显促进了植物生长.

Bioremediation of petroleum-contaminated soil by two strains of fungi and its effect on physiological parameters of plant seedling

Two strains of fungi which were isolated from the petroleum polluted salt alkaloid soil with the petroleum degeneration ability were mixed with humic acid to form the microbial inoculum.Through the pot experiment,the microbial inoculum degeneration capacity was tested by monitoring the petroleum hydrocarbon degeneration rate,the soil dehydrogenation enzyme activity and soil microbial diversity periodly.The results showed that in the case of tested the soil total petroleum hydrocarbon content of 21 000 mg/kg,adding 5% microbial inoculum in the experimented soil pot,the total concentration of petroleum hydrocarbon decreased by 33.13% after 56 days of incubation.In the water cultivating experiment,two types of treatment was designed,that is,plants grown alone(Alfalfa,Canada oats) and two kinds of microorganisms combined with plants respectively.The root dehydrogenation enzyme activity which representing the plant root system vigor and malonydialdehyde(MDA) content in leaves were tested to study promoting effect on plant grow by fungus.The result indicated that with adding 5% microbial inoculum,plant growth were significantly promoted and plant root activity was increased.