| 拮抗水霉菌株的筛选及抑菌蛋白的分离纯化 |
| |
| 引用本文： | 刘莉莉,路福平,刘浩,周闯.拮抗水霉菌株的筛选及抑菌蛋白的分离纯化[J].天津科技大学学报,2011(4):18-21. |
| |
| 作者姓名： | 刘莉莉 路福平 刘浩 周闯 |
| |
| 作者单位： | 工业发酵微生物教育部重点实验室天津科技大学生物工程学院; |
| |
| 基金项目： | 国家科技基础条件平台项目(2005DKA21204-10) |
| |
| 摘 要： | 水霉病是一种广泛存在于水体中的真菌性病原菌,无寄主选择性,目前渔业生产上主要依赖化学药物防治,导致病原菌的抗药性增强.本研究通过平板对峙培养法,筛选到5株具有拮抗活性的菌株.通过硫酸铵盐析、葡聚糖凝胶和离子交换树脂等方式进行分离纯化,从Bacillus subtilis strain TCCC11201发酵液中分离得到一个对水霉病菌具有抑制活性的蛋白,经SDS-PAGE电泳检测,其相对分子质量为2×104.菌株TCCC 11201代谢产生的活性蛋白具有防治水霉病的潜在应用价值.
|
| 关 键 词： | 水霉 拮抗菌 筛选 抑菌蛋白 分离纯化 |
Selection of Antagonistic Bacteria for Saprolegnia parasitica and Isolation of Its Antibiotic Protein |
| |
| LIU Li-li,LU Fu-ping,LIU Hao,ZHOU Chuang.Selection of Antagonistic Bacteria for Saprolegnia parasitica and Isolation of Its Antibiotic Protein[J].Journal of Tianjin University of Science & Technology,2011(4):18-21. |
| |
| Authors: | LIU Li-li LU Fu-ping LIU Hao ZHOU Chuang |
| |
| Institution: | LIU Li-li,LU Fu-ping,LIU Hao,ZHOU Chuang(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China) |
| |
| Abstract: | Saprolegnia parasitica is a pathogenic fungal of the fish,which is prevalence without host selectivity.Recently,it was prevented and treated mainly depend on the chemicals.Five bacterial from the strain bank of our laboratory were sieved out with the dural culture method.A kind of protein was separated out from the fermentation broth of the Bacillus subtilis strain TCCC 11201 which can inhibit the growth of water mold through sephadex gel,ion exchange resin and so on.After detected with SDS-PAGE protein ele... |
| |
| Keywords: | Saprolegnia parasitica antagonistic bacterial screening antibiotic protein separation and purification |
| 本文献已被 CNKI 等数据库收录！ |
|
