| 不同预处理方法对棉秆酶水解的影响 |
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| 引用本文： | 齐临冬,王高升,于孟辉,普春刚.不同预处理方法对棉秆酶水解的影响[J].天津科技大学学报,2011(5):27-31. |
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| 作者姓名： | 齐临冬 王高升 于孟辉 普春刚 |
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| 作者单位： | 天津市制浆造纸重点实验室天津科技大学材料科学与化学工程学院;制浆造纸工程国家重点实验室华南理工大学; |
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| 基金项目： | 天津市高等学校科技发展基金资助项目(20080522); 华南理工大学制浆造纸工程国家重点实验室开放基金资助项目(200924) |
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| 摘 要： | 研究稀硫酸法、亚硫酸法、亚硫酸盐法预处理的化学药品添加量对棉秆酶水解的影响,对预处理前后的棉秆进行扫描电镜观察,并对3种方法进行了比较.在固液比1∶4、温度180,℃、保温20,min的预处理条件下,纤维素酶用量(相对于绝干底物)10 U/g、纤维二糖酶用量(相对于绝干底物)3.6 U/g的酶水解条件下,稀硫酸法预处理在98%浓硫酸添加量为5.52%时,棉秆的酶水解转化率为42.63%;亚硫酸法预处理在亚硫酸添加量7%时,棉秆的酶水解转化率为81.25%;亚硫酸盐法预处理在98%浓硫酸添加量0.92%、亚硫酸氢钠添加量为8%时,棉秆的酶水解转化率为70.06%.
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| 关 键 词： | 棉秆 酶水解 预处理 |
Effects of Different Pretreatment Methods on Enzymatic Hydrolysis of Cotton Stalk |
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| QI Lin-dong,WANG Gao-sheng,YU Meng-hui,PU Chun-gang.Effects of Different Pretreatment Methods on Enzymatic Hydrolysis of Cotton Stalk[J].Journal of Tianjin University of Science & Technology,2011(5):27-31. |
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| Authors: | QI Lin-dong WANG Gao-sheng YU Meng-hui PU Chun-gang |
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| Institution: | QI Lin-dong1,WANG Gao-sheng1,2,YU Meng-hui1,PU Chun-gang1(1.Tianjin Key Laboratory of Pulp and Paper,College of Material Science and Chemical Engineering,Tianjin University of Science & Technology,Tianjin 300457,China,2.State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,China) |
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| Abstract: | Effect of the chemicals dosage of dilute sulfuric acids,ulfurous acid and sulfite pretreatment and enzymatic hy-drolysis of cotton stalk was investigated.The scanning electron microscopic(SEM)analysis of untreated and treated cotton stalk was performed for comparing these methods.Under conditions of liquid ratio 1∶4t,emperature 180,℃ for 20,min,cellulase 10 U/gc,ellobiase 3.6 U/g compared to substrate,enzymatic hydrolysis conversion rate was 42.63% when 5.52% sulfuric acid with mass fraction of 98% for pretreating cotton stalk,enzymatic hydrolysis conversion rate was 81.25% when 7% sulfurous acid for pretreating cotton stalk,and enzymatic hydrolysis conversion rate was 70.06% when 0.92% sulfuric acid with mass fraction of 98% and 8% sodium bisulfite for pretreating cotton stalk. |
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| Keywords: | cotton stalk enzymatic hydrolysis pretreatment method |
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