| 从通道水平研究活性氧对萝卜液泡膜的影响 |
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| 引用本文： | 张朝峰,杨频.从通道水平研究活性氧对萝卜液泡膜的影响[J].山西大学学报(自然科学版),2004,27(2):175-177. |
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| 作者姓名： | 张朝峰 杨频 |
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| 作者单位： | 山西大学,分子科学研究所化学生物学与分子工程教育部重点实验室,山西,太原,030006 |
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| 摘 要： | 用膜片钳全液泡记录方式研究了H2O2对心灵美萝卜(Raphanus satirus L．)液泡膜上SV通道电流的影响．结果表明，H2O2可以抑制SV通道电流，且抑制作用随H2O2浓度的增大而增大，半抑制浓度(IC50)为0.71mmol／L，加入活性氧(ROS)清除剂维生素C不能够消除H2O2的影响，提示H2O2对SV通道的影响是不可逆的．
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| 关 键 词： | 活性氧 SV通道 过氧化氢 膜片钳 离子通道 生物膜 |
| 文章编号： | 0253-2395(2004)02-0175-03 |
| 修稿时间： | 2003年11月14 |
Research at Channel Level on the Effect of Reactive Oxygen Species on Radish Vacuolar Membrane |
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| ZHANG Chaofeng,YANG Pin.Research at Channel Level on the Effect of Reactive Oxygen Species on Radish Vacuolar Membrane[J].Journal of Shanxi University (Natural Science Edition),2004,27(2):175-177. |
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| Authors: | ZHANG Chaofeng YANG Pin |
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| Abstract: | The effects of hydrogen peroxide on slow-vacuolar(SV)ion channels of radish were studied by using the whole-vacuole patch clamp techniques. H_2O_2caused a decrease of SV currents in a concentration-dependent manner. The half-blocking concentration(IC_(50))of H_2O_2 on SVcurrents was 0.71 mmol/L. Added scavenging agent ascorbic acid can not recovery SVcurrents,it suggests that the effects of H_2O_2 on SV currents are irreversible. |
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| Keywords: | SV channel hydrogen peroxide patch clamp |
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