| 门多萨假单胞菌DS04-T对Poly(3HB-co-4HB)的降解研究 |
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| 引用本文： | 李琳琳,高 佳,杨翔华,王战勇.门多萨假单胞菌DS04-T对Poly(3HB-co-4HB)的降解研究[J].河北科技大学学报,2012,33(5):459-463. |
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| 作者姓名： | 李琳琳 高 佳 杨翔华 王战勇 |
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| 作者单位： | 辽宁石油化工大学环境与生物工程学院,辽宁抚顺,113001 |
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| 基金项目： | 国家自然科学基金资助项目（31100099）;辽宁省教育厅科学技术项目（12011060）;辽宁石油化工大学科学基金项目（20llXJJ-025） |
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| 摘 要： | 考察了门多萨假单胞菌DS04-T对Poly(3HB-co-4HB)的降解行为。以Poly(3HB-co-4HB)为唯一碳源,分别考察培养时间、培养温度、摇床转速、装液量、培养基起始pH值、接种量等因素对降解行为的影响。结合正交试验优化获得了菌株的最佳产酶条件:培养时间为28h,培养温度为30℃,培养基初始pH值为7.3,摇床转速为150r/min,培养基装液量为120mL(250mL三角瓶),接种量为1.5%(体积分数),此条件下菌株对Poly(3HB-co-4HB)的降解酶活力可达(26.2±0.7)U.mL-1。
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| 关 键 词： | 门多萨假单胞菌 Poly(3HB-co-4HB) 降解 酶活力 |
| 收稿时间： | 2012/3/5 0:00:00 |
| 修稿时间： | 2012/6/11 0:00:00 |
Degradation of Poly(3HB-co-4HB) by pseudomonas mendocina DS04-T |
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| LI Lin-lin,GAO Ji,YANG Xiang-hua and WANG Zhan-yong.Degradation of Poly(3HB-co-4HB) by pseudomonas mendocina DS04-T[J].Journal of Hebei University of Science and Technology,2012,33(5):459-463. |
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| Authors: | LI Lin-lin GAO Ji YANG Xiang-hua and WANG Zhan-yong |
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| Institution: | (School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun Liaoning 113001, China) |
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| Abstract: | The degradation of Poly（3HB-co-4HB） by pseudomonas mendocina DS04-T was studied. Poly（3HB-co-4HB） was used as the sole carbon source of culture medium. The influence of cultivation time, cultivation temperature, shaking revolu tion, liquid medium volume, initial pH and inoculation content were studied. Combining single factor test and orthogonal test, the optimal degrading enzyme production conditions were chosen as follows： cultivation time is 28 h, cultivation temperature is 30 ℃, shaking revolution is 150 r/rain, initial pH is 7.3, culture medium volume is 120 mL and inoculation content is 1.5% （V/V）. Under the optimized condition, the degradation enzyme activity reaches （26. 220. 7） U · mL-1. |
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| Keywords: | pseudomonas mendocina Poly（3HB-co-4HB） degradation enzyme activity |
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