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高效液相色谱法测定纳豆及纳豆胶囊中的 大豆异黄酮含量
引用本文:张岩,王莉娜.高效液相色谱法测定纳豆及纳豆胶囊中的 大豆异黄酮含量[J].北京工商大学学报(自然科学版),2011,29(1):38-41.
作者姓名:张岩  王莉娜
作者单位:北京燕京啤酒集团公司,技术中心,北京,101300
摘    要:建立了纳豆及纳豆胶囊中大豆异黄酮的高效液相色谱分析方法,该方法重复性及样品稳定性良好.实验对原料黄豆、纳豆和纳豆胶囊样品采用石油醚索氏脱脂后,对固体样品进行乙醇回流提取,分析了4种大豆异黄酮组分,即大豆苷、染料木苷、大豆素和染料木素.结果表明,原料黄豆总异黄酮质量比为1 260 mg/kg,纳豆比原料黄豆总异黄酮含量明显高约30%,纳豆胶囊比原料黄豆总异黄酮含量高约11%.

关 键 词:纳豆  纳豆胶囊  大豆异黄酮  液相色谱法

Determination of Soybean Isoflavones in Natto and Natto Capsule by HPLC
ZHANG Yan and WANG Li-na.Determination of Soybean Isoflavones in Natto and Natto Capsule by HPLC[J].Journal of Beijing Technology and Business University:Natural Science Edition,2011,29(1):38-41.
Authors:ZHANG Yan and WANG Li-na
Institution:ZHANG Yan,WANG Li-na(Technology centre,Beijing yanjing beer group corp,Beijing 101300,China)
Abstract:A high performance liquid chromatographic method was developed for the determination of soybean isoflavones in Natto and Natto Capsule,and the method showed good repeatability and sample stability.Soybean isoflavone were extracted from defatted soybean,Natto and Natto capsule by heating circumfluence method.Four kinds of soybean isoflavone including daidzin,genistin,daidzein and genistein were determined.The results showed that the soybean isoflavones in Natto were 1 260 mg/kg,which were 30% higher than tha...
Keywords:natto  natto capsule  soybean isoflavones  high performance liquid chromatography  
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