PLGA多孔支架降解与成骨细胞相互作用的影响

研究聚丙交酯-乙交酯(PLGA)多孔支架降解和大鼠股骨成骨细胞生理活性之间的相互影响.将大鼠股骨成骨细胞接种于PLGA多孔支架上,观察4周降解时间内成骨细胞增殖活性、碱性磷酸酶(ALP)活性和钙浓度的变化以及PLGA相对分子质量损失、拉伸性能和压缩性能的变化.实验结果表明,支架降解初期,成骨细胞增殖活性较高,ALP活性和分泌钙的浓度较低;支架降解中后期,细胞增殖速度明显减慢,ALP活性和分泌钙的浓度明显下降.接种有细胞的支架降解过程中相对分子质量减小的速度快于对照组,力学拉伸强度和压缩杨氏模量低于对照组.支架材料中后期降解对细胞产生的影响大于降解初期,细胞附着于支架的生理活动加快了支架降解速度.

Study on the Interaction Between Degradation of Poly Porous Scaffolds and Osteoblast (1.School of Life Science, Beijing Institute of Technology, Beijing 100081, China; 2.School of Biology Science & Medical Engineering, Beihang University, Beijing 100191,

To provide data for further researches about the rules of poly(lactide-co-glycolide)(PLGA) porous scaffolds degradation in vivo,the interaction between degradation of PLGA porous scaffolds and osteoblast activities of rat femur is analyzed in this study.Osteoblast of rat femur was planted in PLGA porous scaffolds.During four weeks’ degradation,the properties of osteoblast and PLGA had being observed and measured,including proliferation activities,alkaline phosphatase,calcium concentration,PLGA’s relative molecular mass loss,mechanical tension strength and compression Young modulus.The results show that osteoblasts have high proliferation activity but low ALP activity and calcium concentration at the early stage of degradation.While during later stage of degradation,cells proliferate slowly and ALP activity and calcium concentration decrease remarkably.Relative molecular mass loss rate of scaffolds planted with cells are faster than control group.And mechanical tension strength and compression Young modulus are lower than control group.Above regularities indicate that PLGA porous scaffolds degradation has greater effect on cells during the later stage of degradation than the initial degradation,and the physiological activities of cells adhesion to scaffolds speed up the rate of degradation.