| 污水处理厂出水维甲酸X受体干扰效应研究 |
| |
| 引用本文： | 李剑,孔东东,王子健,马梅.污水处理厂出水维甲酸X受体干扰效应研究[J].北京理工大学学报,2011,31(2):230-235. |
| |
| 作者姓名： | 李剑 孔东东 王子健 马梅 |
| |
| 作者单位： | 李剑,孔东东,LI Jian,KONG Dong-dong(北京师范大学,水科学研究院地下水污染控制与修复教育部工程研究中心,北京,100875);王子健,马梅,WANG Zi-jian,MA Mei(中国科学院,生态环境研究中心环境水质学国家重点实验室,北京,100085) |
| |
| 基金项目： | 国家自然科学基金，中国科学院知识创新工程项目 |
| |
| 摘 要： | 应用重组维甲酸X受体(RXR)基因酵母筛选南方某污水处理厂进水、不同工艺出水的维甲酸干扰活性;结合大鼠肝均浆(S9)体外代谢方法,检测样品间接维甲酸干扰活性.结果表明:进水、出水样品均不能诱导RXR介导酶活性,但在高富集倍数下,样品与空白对照相比显著抑制9-顺维甲酸诱导酶活性,表现出维甲酸拮抗效应;在添加S9体外代谢活化后,所有样品均检出类维甲酸干扰活性,部分样品还检测出抗维甲酸活性,表明城市污水样品中存在大量具有类/抗维甲酸干扰活性的化合物.研究结果证实,现行处理工艺对类/抗维甲酸干扰物有一定的去除效果;重组基因酵母结合S9体外代谢活化体系可用于水环境样品类/抗维甲酸干扰效应的快速筛选.
|
| 关 键 词： | 维甲酸X受体 重组基因酵母 污水 类/抗维甲酸活性 |
| 收稿时间： | 2010/4/21 0:00:00 |
Study on the Retinoid X Receptor (RXR) Disruption of Effluents from Wastewater Treatment Plant |
| |
| LI Jian,KONG Dong-dong,WANG Zi-jian and MA Mei.Study on the Retinoid X Receptor (RXR) Disruption of Effluents from Wastewater Treatment Plant[J].Journal of Beijing Institute of Technology(Natural Science Edition),2011,31(2):230-235. |
| |
| Authors: | LI Jian KONG Dong-dong WANG Zi-jian and MA Mei |
| |
| Institution: | LI Jian1,KONG Dong-dong1,WANG Zi-jian2,MA Mei2(1.Engineering Research Center of Groundwater Pollution Control and Remediation,Ministry of Education,College of Water Science,Beijing Normal University,Beijing 100875,China,2.State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China) |
| |
| Abstract: | The recombinant retinoid X receptor(RXR) gene yeast was conducted to assess the RXR ant/agonistic activities of effluents collected from wastewater treatment plant(WWTP) at a city in south China.Also,a metabolic activation method was introduced based on rat liver S9 for screening indirect RXR ant/agonists activity.The results indicated all of the samples including influent and effluent couldn't induce the enzyme activity.But,compared with blank control,all samples with higher concentration obviously inhibit... |
| |
| Keywords: | retinoid X receptor recombinant gene yeast wastewater RXR ant/agonistic activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《北京理工大学学报》浏览原始摘要信息 |
| 点击此处可从《北京理工大学学报》下载免费的PDF全文 |
|
