三维适形放射治疗犬自发性前列腺增生的扫描电镜观察

目的:研究三维适形放射治疗后前列腺增生组织细胞超微结构的变化,探讨三维适形放射治疗的作用。方法：选择7～15岁老年犬自发性BPH模型10只，随机分为2组。应用3-DCRT直接照射前列腺。以90％的等剂量曲线包绕整个前列腺，单次剂量2Gy，总剂量14Gy，应用6MV-X线完成照射。对照射前、照射后1、3月前列腺组织切片扫描射电镜观察，比较照射前、后组织形态学变化。结果：与照射前比较，照射后扫描电镜下可见，前列腺腺体萎缩，腺体之间间距增大，腺细胞脱落形成空洞。平滑肌萎缩，以纤维组织为主，纤维纤细。结论：3-DCRT可以使腺体和间质萎缩从而缩小前列腺体积改善尿路梗阻和下尿路症状。

Scanning electron microscopic observation of Three-dimensional conformal radiation therapy of canine with spontaneous benign prostatic hyperplasia

Objective: To explore the ultrastructural changes of the prostate after three-dimensional conformal radiation therapy (3-DCRT) of benign prostatic hyperplasia (BPH).Methods: Ten canines of 7 ~ 15 years with spontaneous BPH were chosed as experimental models and were divided for 2 groups randomly. The prostate directly exposed to 3-DCRT.The whole prostate of per animal was the target and sorrounded by 90% isodose curve,and was subjected to irradiation of 6MV X-ray by 2Gy per time and the total dose of 14 Gy. We compared ultrastructural changes of prostate biopsy before and after irradiation for 1 and 3 month with the scanning electron microscopy. Results: Compared with the preirradiation samples, the postirradiation samples had glandular and smooth muscle atrophy, increased space between the glands,glandular cell shedding, and cavity formation.Conclusions: 3-DCRT can make and stroma shink thus reduce the prostate volume and improve urinary tract obstruction and LUTS.